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ONTOGENY OF TRANSCRIPTION PROFILES DURING MOUSE EARLY CRANIOFACIAL DEVELOPMENT
Karoly, E. D., J E. Schmid, AND E S. Hunter III. ONTOGENY OF TRANSCRIPTION PROFILES DURING MOUSE EARLY CRANIOFACIAL DEVELOPMENT. REPRODUCTIVE TOXICOLOGY 19:265-280, (2005).
Using the CD-1 mouse conceptus, we investigated gene expression changes found in vivo from gestational day (GD)8 through GD9 at 6h intervals, and then at 24h intervals through GD11. Data sets were analyzed for patterns in transcriptional expression over a time course as well as to compare the GD9 in vivo mouse conceptus with conceptuses cultured for 24h from GD8 in whole embryo culture (WEC). Our results show that during development from GD8 to GD11, a series of metabolic pathways related to carbohydrate metabolism and energy production, as well as the signal transduction pathways of the MAPK cascade and Wnt signaling were changing. Previous results have shown that WEC successfully sustains morphological development comparable to that in vivo for at least 24h. We report here that, in the absence of morphological malformations following 24h WEC, 329 genes were differentially expressed between in vivo and in vitro developing conceptuses and these genes were primarily associated with transcription factors.
Record Details:Record Type: DOCUMENT (JOURNAL/PEER REVIEWED JOURNAL)
Organization:U.S. ENVIRONMENTAL PROTECTION AGENCY
OFFICE OF RESEARCH AND DEVELOPMENT
NATIONAL HEALTH AND ENVIRONMENTAL EFFECTS RESEARCH LAB
REPRODUCTIVE TOXICOLOGY DIVISION
DEVELOPMENTAL BIOLOGY BRANCH