Science Inventory

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Keely, S., N. Brinkman, E. Wheaton, M. Jahne, S. Siefring, M. Varma, R. Hill, S. Leibowitz, R. Martin, J. Garland, AND Rich Haugland. Geospatial Patterns of Antimicrobial Resistance Genes in the US EPA National Rivers and Streams Assessment Survey. ENVIRONMENTAL SCIENCE & TECHNOLOGY. American Chemical Society, Washington, DC, 56(21):14960–14971, (2022). https://doi.org/10.1021/acs.est.2c00813

Keely, S., N. Brinkman, E. Wheaton, M. Jahne, S. Siefring, M. Varma, Ryan A Hill, S. Leibowitz, R. Martin, J. Garland, AND Rich Haugland. Large-scale Patterns of Antimicrobial Resistance Genes and Fecal Indicator Bacteria in United States Rivers and Streams. 2021 US EPA National Recreational Water Quality Workshop, Virtual, April 06 - 08, 2021.

Briggs, S., M. Sivaganesan, O. Shanks, R. Haugland, S. Siefring, M. Varma, K. Oshima, A. Dufour, S. Nappier, AND B. Schnitker. Derivation of the equivalent qPCR value for Escherichia coli to existing culture-based water quality standards for monitoring beaches in Michigan. IWA 20th International Symposium on Health Related Water Microbiology, Vienna, AUSTRIA, September 15 - 20, 2019.

Haugland, Rich, S. Siefring, E. Anneken, M. Varma, S. Keely, R. Mitchell, AND S. Lehmann. Low Frequencies of Interference to EPA Quantitative Polymerase Chain Reaction (qPCR) Methods for Microbial Water Quality Monitoring in U.S. Rivers and Streams and Coastal Waters. 2018 UNC Water Microbiology Conference, Chapel Hill, NC, May 22 - 24, 2018.

Sivaganesan, Mano, M. Varma, S. Siefring, AND Rich Haugland. Quantification of plasmid DNA standards for U.S. EPA fecal indicator bacteria qPCR methods by droplet digital PCR analysis. JOURNAL OF MICROBIOLOGICAL METHODS. Elsevier Science Ltd, New York, NY, 152:135-142, (2018). https://doi.org/10.1016/j.mimet.2018.07.005

Napier, M., Rich Haugland, C. Poole, A. Dufour, J. Stewart, D. Weber, M. Varma, J. Lavender, AND Tim Wade. Exposure to human-associated fecal indicators and self-reported illness among swimmers at recreational beaches: A cohort study. ENVIRONMENTAL HEALTH. Academic Press Incorporated, Orlando, FL, 16(1):103, (2017). https://doi.org/10.1186/s12940-017-0308-3

Paar, J., M. Doolittle, M. Varma, S. Siefring, K. Oshima, AND Rich Haugland. Development and evaluation of a culture-independent method for source determination of fecal wastes in surface and storm waters using reverse transcriptase-PCR detection of FRNA coliphage genogroup gene sequences. JOURNAL OF MICROBIOLOGICAL METHODS. Elsevier Science Ltd, New York, NY, 112(2):28-35, (2015).

Sivaganesan, Mano, S. Siefring, M. Varma, AND Rich Haugland. Comparison of Enterococcus quantitative polymerase chain reaction analysis results from midwest U.S. river samples using EPA Method 1611 and Method 1609 PCR reagents. JOURNAL OF MICROBIOLOGICAL METHODS. Elsevier Science Ltd, New York, NY, 101(6):9-17, (2014).

Green, H., Rich Haugland, M. Varma, H. Millen, M. Borchardt, K. Field, W. Walters, R. Knight, Mano Sivaganesan, C. Kelty, AND O. Shanks. Improved HF183 quantitative real-time PCR assay for characterization of human fecal pollution in ambient surface water samples. APPLIED AND ENVIRONMENTAL MICROBIOLOGY. American Society for Microbiology, Washington, DC, 80(10):3086-3094, (2014).

Haugland, Rich, S. Siefring, M. Varma, A. Dufour, K. Brenner, Tim Wade, E. Sams, S. Cochran, S. Braun, AND Mano Sivaganesan. Standardization of enterococci density estimates by EPA qPCR methods and comparison of beach action value exceedances in river waters with culture methods. JOURNAL OF MICROBIOLOGICAL METHODS. Elsevier Science Ltd, New York, NY, 105(7):59-66, (2014).

Sivaganesan, Mano, M. Varma, AND Rich Haugland. Comparison of Enterococcus qPCR analysis results from fresh and marine water samples on two real-time instruments - poster. Presented at ISME14, International Society for Microbial Ecology, Copenhagen, DENMARK, August 19 - 24, 2012.

SIVAGANESAN, M., M. VARMA, AND R. A. HAUGLAND. Comparison of Enterococcus qPCR analysis results from fresh and marine waters on two real-tme instruments. ANALYTICAL BIOCHEMISTRY. Academic Press Incorporated, Orlando, FL, 430(1):68-74, (2012).

Haugland, Rich, M. Varma, AND L. Wymer. Detection and quantification limits of the EPA Enterococcus qPCR method. 12th Annual Great Lakes Beach Asssociation Conference, Mackinac Island, MI, October 16 - 18, 2012.

KELTY, C. A., M. VARMA, M. SIVAGANESAN, R. A. HAUGLAND, AND O. C. SHANKS. Distribution of Genetic Marker Concentrations for Fecal Indicator Bacteria in Sewage and Animal Feces. APPLIED AND ENVIRONMENTAL MICROBIOLOGY. American Society for Microbiology, Washington, DC, 78(12):4225-4232, (2012).

HAUGLAND, R. A., S. SIEFRING, J. Lavender, AND M. VARMA. Influences of sample interference and interference controls on quantification of enterococci fecal indicator bacteria in surface water samples by the qPCR method. WATER RESEARCH. Elsevier Science Ltd, New York, NY, 46(18):5989-6001, (2012).

SHANKS, O. C., MANO SIVAGANESAN, L. PEED, C. A. KELTY, A. D. Blackwood, M. R. Greene, R. T. Noble, R. N. Bushon, E. A. Stelzer, J. Kinzelman, T. Anan'eva, C. Sinigalliano, D. Wanless, J. Griffith, Y. Cao, S. Weisberg, V. J. Harwood, C. Staley, K. OSHIMA, M. VARMA, AND R. A. HAUGLAND. Interlaboratory Comparison of Real-time PCR Protocols for Quantification of General Fecal Indicator Bacteria. ENVIRONMENTAL SCIENCE AND TECHNOLOGY. ACS Publications, Washington, DC, 46(2):945-953, (2012).

SIVAGANESAN, M., S. SIEFRING, M. VARMA, AND R. A. HAUGLAND. MPN estimation of qPCR target sequence recoveries from whole cell calibrator samples. JOURNAL OF MICROBIOLOGICAL METHODS. Elsevier Science Ltd, New York, NY, 87(3):3235-3249, (2011).

WYMER, L. J., K. OSHIMA, J. PAAR, M. Doolittle, J. Lavender, M. VARMA, AND R. A. HAUGLAND. Effects of Holding Time, Storage, and the Preservation of Samples on Sample Integrity for the Detection of Fecal Indicator Bacteria by Quantitative Polymerase Chain Reaction (qPCR)-based assays. U.S. Environmental Protection Agency, Washington, DC, EPA/600/R-10/150, 2010.

HAUGLAND, R. A., M. VARMA, M. SIVAGANESAN, C. A. KELTY, L. PEED, AND O. C. SHANKS. Evaluation of genetic markers from the 16S rRNA gene V2 region for use in quantitative detection of selected Bacteroidales species and human fecal waste by real time PCR. SYSTEMATIC AND APPLIED MICROBIOLOGY. Elsevier Science Ltd, New York, NY, 33(6):348-357, (2010).

SHANKS, O. C., K. WHITE, C. A. KELTY, M. SIVAGANESAN, J. BLANNON, M. MECKES, M. VARMA, AND R. A. HAUGLAND. Performance of PCR-based assays targeting Bacteroidales genetic markers of human fecal pollution in sewage and fecal samples. ENVIRONMENTAL SCIENCE AND TECHNOLOGY. American Chemical Society, Washington, DC, 44(16):6281-6288, (2010).

KELTY, C. A., K. M. WHITE, J. C. BLANNON, M. C. MECKES, O. C. SHANKS, M. SIVAGANESAN, M. VARMA, AND R. A. HAUGLAND. Single Laboratory Comparison of Quantitative Real-time PCR Assays for the Detection of Fecal Pollution. Presented at American Society for Microbiology 110th General Meeting, San Diego, CA, May 23 - 27, 2010.

HAUGLAND, R. A., M. VARMA, R. OSHIRO, J. Parr, AND M. Doolittle. Comparison of Enterococcus qPCR analysis results from fresh and marine water samples on two real-time instruments - . Presented at National Beaches Conference 2009, Huntington Beach, CA, April 20 - 22, 2009.

SHANKS, O. C., K. M. WHITE, C. A. KELTY, S. L. HAYES, M. SIVAGANESAN, M. Jenkins, M. VARMA, AND R. A. HAUGLAND. Host Distribution of Bacteroidales Genetic Markers of Bovine Fecal Pollution. AMERICAN SOCIETY FOR MICROBIOLOGY. ASM Press, Washington, DC, , ,, (2009).

SHANKS, O. C., C. A. KELTY, M. SIVAGANESAN, M. VARMA, AND R. A. HAUGLAND. Quantitative PCR for Genetic Markers of Human Fecal Pollution. APPLIED AND ENVIRONMENTAL MICROBIOLOGY. American Society for Microbiology, Washington, DC, 75(17):5507-5513, (2009).

SHANKS, O. C., C. A. KELTY, M. SIVAGANESAN, M. VARMA, AND R. A. HAUGLAND. Quantitative PCR for genetic markers of human fecal pollution . APPLIED AND ENVIRONMENTAL MICROBIOLOGY. American Society for Microbiology, Washington, DC, 75(17):5507-5513, (2009).