Office of Research and Development Publications

Records 1 to 22 of 22

Paar, J., M. Doolittle, M. Varma, S. Siefring, K. Oshima, AND Rich Haugland. Development and evaluation of a culture-independent method for source determination of fecal wastes in surface and storm waters using reverse transcriptase-PCR detection of FRNA coliphage genogroup gene sequences. JOURNAL OF MICROBIOLOGICAL METHODS. Elsevier Science Ltd, New York, NY, 112(2):28-35, (2015).
Haugland, Rich, S. Siefring, M. Varma, A. Dufour, K. Brenner, Tim Wade, E. Sams, S. Cochran, S. Braun, AND Mano Sivaganesan. Standardization of enterococci density estimates by EPA qPCR methods and comparison of beach action value exceedances in river waters with culture methods. JOURNAL OF MICROBIOLOGICAL METHODS. Elsevier Science Ltd, New York, NY, 105(7):59-66, (2014).
Sivaganesan, Mano, S. Siefring, M. Varma, AND Rich Haugland. Comparison of Enterococcus quantitative polymerase chain reaction analysis results from midwest U.S. river samples using EPA Method 1611 and Method 1609 PCR reagents. JOURNAL OF MICROBIOLOGICAL METHODS. Elsevier Science Ltd, New York, NY, 101(6):9-17, (2014).
Haugland, R A., S D. Siefring, L J. Wymer, K Brenner, AND A P. Dufour. COMPARISON OF ENTEROCOCCUS MEASUREMENTS IN FRESHWATER AT TWO RECREATIONAL BEACHES BY QUANTITATIVE POLYMERASE CHAIN REACTION AND MEMBRANE FILER CULTURE ANALYSIS. WATER RESEARCH 39(4):559-568, (2005).
SIVAGANESAN, M., S. SIEFRING, M. VARMA, AND R. A. HAUGLAND. MPN estimation of qPCR target sequence recoveries from whole cell calibrator samples. JOURNAL OF MICROBIOLOGICAL METHODS. Elsevier Science Ltd, New York, NY, 87(3):3235-3249, (2011).
HAUGLAND, R. A., S. SIEFRING, J. Lavender, AND M. VARMA. Influences of sample interference and interference controls on quantification of enterococci fecal indicator bacteria in surface water samples by the qPCR method. WATER RESEARCH. Elsevier Science Ltd, New York, NY, 46(18):5989-6001, (2012).
CHERN, E. C., S. SIEFRING, J. PAAR, M. Doolittle, AND R. A. HAUGLAND. Comparison of quantitative PCR assays for Escherichia coli targeting ribosomal RNA and single copy genes. Letters in Applied Microbiology. Blackwell Publishing, Malden, MA, 52(3):298-306, (2012).
HAUGLAND, R. A., S. SIEFRING, M. VARMA, AND L. J. WYMER. Laboratory Evaluations of the Enterococcus qPCR Method for Recreational Water Quality Testing: Method Performance and Sources of Uncertainty in Quantitative Measurements. Presented at 108th General Meeting of the American Society for Microbiology, Boston, MA, June 01 - 05, 2008.
SIEFRING, S., R. A. HAUGLAND, AND L. J. WYMER. Evaluation of Commercial Cell Preparations as Sources of Calibration Standards for Real-Time qPCR Analysis of Enterococci in Recreational Waters. Presented at Great Lakes Beaches Association Annual Meeting, Porter, IN, September 15 - 17, 2008.
SIEFRING, S., E. ATIKOVIC, R. A. HAUGLAND, M. SIVAGANESAN, AND O. C. SHANKS. INTERNAL AMPLIFICATION CONTROL FOR USE IN QUANTITATIVE POLYMERASE CHAIN REACTION FECAL INDICATOR BACTERIA ASSAYS. Presented at Ohio Branch of the American Society for Microbiology Annual Meeting, Canton, OH, April 13 - 14, 2007.
HAUGLAND, R. A., S. SIEFRING, M. VARMA, E. ATIKOVIC, K. P. BRENNER, A. P. DUFOUR, AND L. J. WYMER. EVALUATION OF MATRIX EFFECTS FROM NATIONWIDE INLAND SURFACE WATERS ON QUANTITATION POLYMERASE CHAIN REACTION ANALYSIS FOR FECAL INDICATOR BACTERIA. Presented at National Beaches Conference, Niagra Falls, NY, October 11 - 13, 2006.
SIEFRING, S., L. J. WYMER, AND R. A. HAUGLAND. NEW TARGET AND CONTROL ASSAYS FOR QUANTITATIVE POLYMERASE CHAIN REACTION (QPCR) ANALYSIS OF ENTEROCOCCI IN WATER. Presented at American Society for Microbiology General Meeting, Atlanta, GA, June 05 - 09, 2005.
Siefring, S D., J Santo Domingo, N Brinkman, AND R A. Haugland. DETECTION OF FECAL ENTEROCOCCI USING A REAL TIME PCR METHOD. Presented at American Society of Microbiology, Orlando, FL, May 19-24, 2001.
Brenner, K P., S D. Siefring, AND S L. Brown. COMPARISON OF MENTEROCOCCUS AGAR AND THE U.S. ENVIRONMENTAL PROTECTION AGENCY-RECOMENDED ENTEROCOCCI METHODS, ME AND MEI AGAR. Presented at American Society of Microbiology, Los Angeles, CA, May 21-24, 2000.
Brown, S, J Santo Domingo, S D. Siefring, AND R A. Haugland. USE OF TAQMAN TO ENUMERATE ENTEROCOCCUS FAECALIS IN WATER. Presented at American Society of Microbiology, Orlando, FL, May 19-24, 2001.
Haugland, R A., S D. Siefring, L J. Wymer, K Brenner, AND A P. Dufour. COMPARISON OF ENTEROCCOCUS DENSITIES DETERMINED BY CULTURE AND QPCR ANALYSES IN WATER SAMPLES FROM TWO RECREATION BEACHES. Presented at New England Association of Environmental Biologists (NEAEB), Hancock, MA, March 17-19, 2004.
Haugland, R A., K P. Brenner, A P. Dufour, AND S D. Siefring. THE NEED FOR SPEED - RAPID METHODOLOGIES TO DETERMINE BATHING BEACH WATER QUALITY. Presented at Science Forum 2003, Washington, DC, May 5-7, 2003.
Haugland, R A., S D. Siefring, K Brenner, A P. Dufour, J. A. Griffith, K. Schiff, S. B. Weisberg, J. Paar, AND K. G. Field. RAPID MEASUREMENT OF BACTERIAL FECAL POLLUTION INDICATORS AT RECREATIONAL BEACHES BY QUANTITATIVE POLYMERASE CHAIN REACTION. Presented at National Beaches Conference, San Diego, CA, October 13-15, 2004.
VARMA, M., S. SIEFRING, E. ATIKOVIC, L. J. WYMER, AND R. A. HAUGHLAND. MONITORING FECAL INDICATOR BACTERIA WITH ALTERNATIVE REAL-TIME PCR INSTRUMENTS TO ASSESS HEALTH RISKS ASSOCIATED WITH RECREATIONAL WATER USE. Presented at 2006 EPA Science Forum, Washington, DC, May 16 - 18, 2006.
HAUGLAND, R. A., S. SIEFRING, M. VARMA, E. ATIKOVIC, AND L. J. WYMER. COMPARISON OF REAL-TIME PCR FECAL BACTERIA MEASUREMENTS IN RECREATIONAL WATERS USING DIFFERENT INSTRUMENTS AND REAGENT SYSTEMS. Presented at 106th General Meeting of the American Society for Microbiology, Orlando, FL, May 21 - 25, 2006.
BRENNER, K. P., R. L. CALDERON, A. P. DUFOUR, R. A. HAUGLAND, E. A. SAMS, T. J. WADE, AND S. SIEFRING. RAPID HEALTH-BASED METHOD FOR MEASURING MICROBIAL INDICATORS OF RECREATIONAL WATER QUALITY - 2006 EPA SCIENCE FORUM. Presented at 2006 EPA Science Forum, Washington, DC, May 16 - 18, 2006.
Santo Domingo, J, S D. Siefring, AND R A. Haugland. REAL-TIME PCR METHOD TO DETECT ENTEROCOCCUS FAECALIS IN WATER. BIOTECHNOLOGY LETTERS 25(3):261-265, (2003).