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Records with

  • Keyword = ’homeland security’ AND 'indoor outdoor decontamination' AND biological NOT (presentation OR poster).
Records 1 to 25 of 317

2021
Decontamination of soil contaminated at the surface with Bacillus anthracis spores using dry thermal treatment
(JOURNAL) [Published : Feb 15, 2021]
In the event of a wide-area release of Bacillus anthracis spores, soils and other outdoor materials may become contaminated with the biological agent. A study was conducted to assess the in-situ remediation of soil using a dry thermal treatment approach to inactivate a B. anthrac...
Literature Search and Review for Sampling, Analysis, and Decontamination of Biological Warfare Agent-Contaminated Maritime Vessels
(PUBLISHED REPORT) [Published : Feb 25, 2021]
This report summarizes the current scientific literature on remediation-related activities associated with US Coast Guard vessels and other assets. It also provides descriptions of research gaps and needs for consideration by US Coast Guard and other federal agencies to improve o...
2020
Comparison of surface sampling methods for an extended duration outdoor biological contamination study
(JOURNAL) [Published : Jun 24, 2020]
Bacillus anthracis, the causative agent for anthrax, is a dangerous pathogen to humans and has a history as a bioterrorism agent. While sampling methods have been developed and evaluated for characterizing and clearing contaminated indoor sites, the performance of these sampling ...
Compatibility of Electronic Equipment and Other Materials with Peracetic Acid Fog and Low Concentration Hydrogen Peroxide Vapor
(PUBLISHED REPORT) [Published : Aug 14, 2020]
The purpose of this study was to determine the impacts of PAA fog and LCHPV on representative indoor materials and electronic equipment. The fogging of PAA solution caused visually observed appearance changes (e.g., discoloration, oxidation, residue) on the copper, low-carbon ste...
Dispersion Modeling Systems Relevant to Homeland Security Preparedness and Response
(PUBLISHED REPORT) [Published : Oct 21, 2020]
Atmospheric dispersion modeling is one tool that can be used for effective emergency preparation or response from hazardous chemical, biological, radiological, nuclear, and explosive (CBRNe) releases, especially in urban areas where population densities are high and wind flow bec...
EVALUATING THE ENVIRONMENTAL PERSISTENCE AND INACTIVATION OF MS2 BACTERIOPHAGE AND THE PRESUMED EBOLA VIRUS SURROGATE PHI6 USING LOW CONCENTRATION HYDROGEN PEROXIDE VAPOR
(JOURNAL) [Published : Mar 17, 2020]
The phages were inoculated onto six material types (both porous and nonporous), with and without the presence of human whole blood. The inoculated materials were then exposed to either a high or low concentration of HPV, and the phages were recovered from the materials at several...
EVALUATION OF ALTERED ENVIRONMENTAL CONDITIONS AS A DECONTAMINATION APPROACH FOR NONSPORE-FORMING BIOLOGICAL AGENTS
(JOURNAL) [Published : Apr 01, 2020]
The purpose of this study was to evaluate the effects of altered environmental conditions on the persistence of Francisella tularensis bacteria and Venezuelan Equine Encephalitis virus, on two material types. Results from this study provide information for inactivation of non-spo...
Inactivation of Bacillus anthracis and Bacillus atrophaeus Spores on Surfaces with Ultraviolet Light Produced Via Either Light Emitting Diodes or a Low-Pressure Mercury Vapor Lamp
(JOURNAL) [Published : Jul 21, 2020]
Journal article; Aims: To obtain data on the efficacy of two ultraviolet light (UVC) technologies for inactivation of Bacillus anthracis Ames and Bacillus atrophaeus spores on surfaces.
Literature Search and Review for Sampling, Analysis, and Decontamination of Chemical Warfare Agent - Contaminated Maritime Vessels
(PUBLISHED REPORT) [Published : Dec 31, 2020]
The USCG protects and defends more than 100,000 miles of U.S. coastline and inland waterways. To this end, the USCG may be responsible for countering and responding to incidents involving weapons of mass destruction, including biological warfare agents (BWAs) and chemical warfare...
OPTIMIZATION OF A RAPID VIABILITY POLYMERASE CHAIN REACTION (RV-PCR) PROTOCOL FOR DETECTION OF FRANCISELLA TULARENSIS IN WATER SAMPLES
(PUBLISHED REPORT) [Published : Mar 18, 2020]
This is a final report for the project on Optimization of a Rapid Viability (RV) PCR Protocol for Detection of Francisella tularensis in water. During a tularemia bioterrorism incident or a natural outbreak, the water system can be contaminated. This pathogen survives for many da...
Summary of hydrogen peroxide vapor generation using off-the-shelf items and equipment for the disinfection of spaces and materials
(SUMMARY) [Published : Sep 29, 2020]
The tech brief also provides information on how these devices could be operated so that a sufficient dose of hydrogen peroxide vapor is generated for disinfection purposes.
Ten Questions Concerning the Implications of Carpet on Indoor Chemistry and Microbiology
(JOURNAL) [Published : Mar 01, 2020]
Carpet and rugs currently represent about half of the United States flooring market and offer many benefits as a flooring type. How carpets influence our exposure to both microorganisms and chemicals in indoor environments has important health implications, but is not well unders...
The Use of Ozone Gas for the Inactivation of Bacillus anthracis and Bacillus subtilis Spores on Building Materials
(JOURNAL) [Published : May 21, 2020]
A study was conducted to assess the efficacy of ozone gas in inactivating spores of both Bacillus anthracis and Bacillus subtilis inoculated onto six building materials (glass, wood, carpet, laminate, galvanized metal, and wallboard paper). Testing conditions consisted of ozone g...
Washing/Drying of Clothing Contaminated with a Biological Agent Such as Bacillus anthracis
(PUBLISHED REPORT) [Published : Sep 30, 2020]
The study was conducted in four phases, each using viable spores of Bacillus atrophaeus var. globigii (Bg), a surrogate for Ba. Phase I and II were part of a bench-scale study using a Lab Line Incubator-Orbital Shaker to simulate a washing machine. The experiment was conducted us...
2019
2018 EPA International Decontamination Research and Development Conference
(SUMMARY) [Published : May 28, 2019]
On May 8-10, 2018, the U.S. Environmental Protection Agency’s (EPA’s) Office of Research and Development’s National Homeland Security Research Center (ORD/NHSRC) hosted the 10th U.S. EPA International Decontamination Research and Development Conference at the EPA facilities in Re...
A Bench-Scale Procedure for Evaluating Chlorine Bleach Inactivation of Bacillus Spores in Wash Water from a Cleanup of a Site with Biothreat Agents
(PUBLISHED REPORT) [Published : May 02, 2019]
This report describes a streamlined bench scale procedure which has been developed for testing the efficacy of chlorine bleach for the inactivation of Bacillus spores in wash water originating from a cleanup of a site contaminated with Bacillus anthracis spores.
Chlorine Exposure During a Biological Decontamination Study in a Mock Subway Tunnel
(JOURNAL) [Published : Jul 12, 2019]
The Underground Transport Restoration (UTR) Operational Technology Demonstration (OTD) was a full-scale study focused on remediation of a subway system after contamination with a Bacillus anthracis (Ba) surrogate (Bacillus atrophaeus, subspecies globigii [Bg]). The study involved...
Colony-Forming Unit Spreadplate Assay versus Liquid Culture Enrichment-Polymerase Chain Reaction Assay for the Detection of Bacillus Endospores in Soils
(JOURNAL) [Published : Dec 21, 2019]
This peer reviewed journal article summarizes research on comparison of an enrichment-PCR assay compared to a processing protocol for soil samples potentially contaminated with Bacillus anthracis
Evaluation of Analytical Methods for the Detection of Bacillus Anthracis Spores: Compatibility with Real-World Samples Collected from Outdoor and Subway Surfaces
(PUBLISHED REPORT) [Published : Aug 20, 2019]
This effort sought to assess the impact of potential real-world interferents (RWIs) present in Sponge-Stick and Vacuum Cassettes (VCF) samples on current culture and molecular analysis methods. Having an assessment of the impact, if any, will help EPA understand limitations of th...
Feasibility Study for Reuse of Activated Carbon for the Capture of Methyl Bromide Used for Decontamination
(PUBLISHED REPORT) [Published : May 20, 2019]
In the present study, we quantified how well an activated carbon (AC) sample maintained its adsorption capacity for Methyl bromide (MeBr) over several adsorption/desorption cycles, using three different gas conditions. During the adsorption phase, the AC was exposed to a feed gas...
Impact of Sporicidal Fumigation with Methyl Bromide or Methyl Iodide on Electronic Equipment
(JOURNAL) [Published : Feb 01, 2019]
The effect of sporicidal fumigation with methyl bromide or methyl iodide on the functionality of electronic equipment was evaluated using desktop computers as surrogates for valuable electronic equipment under target conditions of 200–250 mg/L fumigant for 48 hours at 24–30 °C an...
RAPID VIABILITY POLYMERASE CHAIN REACTION METHOD FOR DETECTION OF FRANCISELLA TULARENSIS
(JOURNAL) [Published : Nov 01, 2019]
Francisella tularensis, which causes potentially fatal tularemia, has been considered an attractive agent of bioterrorism and biological warfare due to its low infectious dose, reported environmental persistence, and ability to be transmitted to humans via multiple exposure route...
Review of Decontamination Techniques for the Inactivation of Bacillus anthracis and Other Spore-Forming Bacteria Associated with Building or Outdoor Materials
(JOURNAL) [Published : Mar 22, 2019]
Although the military has been developing decontamination methods for their purposes for decades, research conducted or funded by nondefense government agencies with a focus on the decontamination of civilian facilities had been minimal prior to 2001. That changed following the i...
SAMPLE ANALYSIS OF NATIVE AIR FILTERS FOR CHARACTERIZATION AND EXTENT MAPPING OF BIOLOGICAL INCIDENTS
(PUBLISHED REPORT) [Published : Aug 30, 2019]
Following a biological contamination incident, the spatial extent of the contamination should be determined using established sampling and analytical methods such as culture or RV-PCR. A previous study demonstrated that a sampling strategy involving native air samplers could be i...
Sample Processing Method for Ricin Analysis
(SUMMARY) [Published : Jan 30, 2019]
Technical Brief