Main Title |
RT-PCR protocols / |
Other Authors |
|
Publisher |
Humana Press, |
Year Published |
2002 |
OCLC Number |
48957956 |
ISBN |
0896038750; 9780896038752 |
Subjects |
Polymerase chain reaction--Laboratory manuals ;
Reverse Transcriptase Polymerase Chain Reaction ;
Polymerase kettingreactie ;
Wetenschappelijke technieken ;
Methode--(DE-588)4038971-6 ;
Polymerase-Kettenreaktion--(DE-588)4256726-9 ;
PCR ;
DNA POLIMERASES ;
RNA MENSAGEIRO ;
Reverse Transcriptase Polymerase Chain Reaction--Laboratory Manuals ;
R eaction en cha ine de la polym erase--Manuels de laboratoire ;
PCR--larpcal ;
DNA POLIMERASES--larpcal ;
RNA MENSAGEIRO--larpcal
|
Internet Access |
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
ESBM |
QP606.D46R8 2002 |
|
CPHEA/PESD Library/Corvallis,OR |
12/12/2003 |
|
Collation |
xiv, 378 pages : illustrations ; 24 cm. |
Notes |
Includes bibliographical references and index. |
Contents Notes |
Rt-pcr in biomedicine. Opportunities arising from the new accessibility of mrna / J. O'Connell -- The basics of rt-pcr. Some practical considerations / J. O'Connell -- Using the quantitative competitive rt-pcr technique to analyze minute amounts of different mrnas in small tissue samples / S. Greber-Platzer [and others] -- Detection of mrna expression and alternative splicing in a single cell / T. Kumazaki -- Nested rt-pcr. Sensitivity controls are essential to determine the biological significance of detected mrna / T. Goode [and others] -- Quantitative rt-pcr. A review of current methodologies / C. Joyce -- Rapid development of a quantitative-competitive (qc) rt-pcr assay using a composite primer approach / J. O'Connell [and others] -- Quantitation of gene expression by rt-pcr and hplc analysis of pcr products / F. Bachmair, C.G. Huber and G. Daxenbichler -- Time-resolved fluorometric detection of cytokine mrnas amplified by rt-pcr / K. Nieminen [and others] -- Mimic-based rt-pcr quantitation of substance p mrna in human mononuclear phagocytes and lymphocytes / J.P. Lai, S.D. Douglas and W.Z. Ho -- Detection and quantification of the hepatitis c viral genome / L.J. Fanning -- Semi-quantitative detection of hepatitis c virus rna by "real-time" rt-pcr / J.F. Schlaak -- Rt-pcr for the assessment of genetically heterogenous populations of the hepatitis c virus / B. Mullan [and others] -- In situ immuno-pcr. A newly developed method for highly sensitive antigen detection in situ / Y. Cao -- Rt-pcr from laser-capture microdissected samples / T. Crnogorac-Jurcevic, T.O. Nielsen and N.R. Lemoine -- Mycobacterium paratuberculosis detected by nested pcr in intestinal granulomas isolated by lcm in cases of crohn's disease / P. Ryan [and others] -- Rt-pcr-based approaches to generate probes for mrna detection by in situ hybridization / J. O'Connell -- Amplified rna for gene array hybridizations / V.I. Shustova and S.J. Meltzer -- Semi-quantitative determination of differential gene expression in primary tumors and matched metastases by rt-pcr. Comparison with other methods / B. Mann and C. Hanski -- Detection of single nucleotide polymorphisms using a non-isotopic rnase cleavage assay / F. Waldron-Lynch [and others] -- Detection of clonally expanded t-cells by rt-pcr-sscp and nucleotide sequencing of t-cell receptor beta-cdr3 regions / M.S. Kurokawa, K. Nishioka and T. Kato -- Generation of scfv from a phage display mini-library derived from tumor-infiltrating b-cells / N. Gruel [and others] -- Generation of murine scfv intrabodies from b-cell hybridomas / C.H. Nam, S. Moutel and J.L. Teillaud -- Quantitation of mrna levels by rt-pcr in cells purified by facs. Application to peripheral cannabinoid receptors in leukocyte subsets / J. Marchand and P. Carayon -- Detection of anti-sense rna transcripts by anti-sense rt-pcr / M.C. Yeung and A.S. Lau -- Rt-pcr in cdna library construction / V. Healy -- An rt-pcr-based protocol for the rapid generation of large, representative cdna libraries for expression screening / J. O'Connell. |
Place Published |
Totowa, N.J. : |
Title Ser Add Ent |
Methods in molecular biology (Clifton, N.J.) ; |
PUB Date Free Form |
c2002. |
Series Title Untraced |
Methods in molecular biology; v. 193 |
BIB Level |
m |
Cataloging Source |
OCLC/T |
LCCN |
2002190221 |
Merged OCLC records |
174055734 |
OCLC Time Stamp |
20031208181212 |
Language |
eng |
Origin |
OCLC |
Type |
CAT |
OCLC Rec Leader |
01338cam 22003974a 45020 |