||Plasmid Expression and Maintenance during Long-Term Starvation-Survival of Bacteria in Well Water.
Caldwell, B. A. ;
Ye, C. ;
Griffiths, R. P. ;
Moyer, C. L. ;
Morita, R. Y. ;
||Oregon State Univ., Corvallis.;Corvallis Environmental Research Lab., OR.
Aquatic microbiology ;
Water wells ;
Escherichia coli ;
Gene expression ;
||Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy.
||Strains of enteric bacteria and pseudomonads containing plasmid R388::Tn1721(Tpr, Tcr) or pR0101 (Hgr, Tcr) were starved for over 250 days in sterile well water to evaluate effects of starvation-survival on plasmid expression and maintenance. Viable populations dropped to between approximately 0.1 and 1% of the initial populations. Escherichia coli(pR0101) and Pseudomonas cepacia (pR0101) lost both viability and plasmid expression at a lower rate than strains containing R388::Tn1721. Three patterns of host-plasmid interaction were detected: (i) no apparent loss of plasmid expression, (ii) loss of plasmid expression on initial recovery with subsequent expression upon resuscitation, and (iii) loss of capability to produce functional plasmid resistance. (Copyright (c) 1989, American Society for Microbiology.)
||Pub. in Applied and Environmental Microbiology, v55 n8 p1860-1864 Aug 89. See also PB89-214415. Sponsored by Corvallis Environmental Research Lab., OR.
|NTIS Title Notes
||Reprint: Plasmid Expression and Maintenance during Long-Term Starvation-Survival of Bacteria in Well Water.
||PC A02/MF A01