||Olfactory Toxicity of Beta,Beta'-Iminodipropionitrile in the Rat.
Genter, M. B. ;
Llorens, J. ;
O'Callaghan, J. P. ;
Peele, D. B. ;
Morgan, K. T. ;
||Health Effects Research Lab., Research Triangle Park, NC. Neurotoxicology Div. ;North Carolina State Univ. at Raleigh. Dept. of Toxicology. ;ManTech Environmental Technology, Inc., Research Triangle Park, NC. ;Chemical Industry Inst. of Toxicology, Research Triangle Park, NC.
Olfactory bulb ;
Dose-response relationships ;
Glial fibrillary acidic protein ;
Time factors ;
Binding sites ;
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||Following a pilot study which revealed olfactory epithelial degeneration induced by beta,beta'-iminodipropionitrile (IDPN), dose-response and time-course analyses were undertaken to further characterize the effects of IDPN on the olfactory system. Male rats were sacrificed at multiple time points ranging from 24 hrs after a single dose to 56 days after three consecutive daily doses of IDPN (0-400 mg/kg i.p.). Nasal cavities were fixed, decalcified and embedded in paraffin; 5 micrometer sections were stained with hematoxylin and eosin, middle neurofilament protein antibody or olfactory marker protein antiserum. Olfactory bulbs were removed for slot blot analyses of glial fibrillary acidic protein, synapsin 1 and p38. Twenty-four hours after treatment with 200 or 400 mg/kg IDPN, there was severe, highly site-specific mucosal degeneration in the dorsal-medial nasal cavity; regeneration was incomplete 8 weeks later. IDPN increased olfactory bulb glial fibrillary acidic protein, peaking 7 days after three daily 400 mg/kg doses, and remaining significantly elevated 8 weeks after treatment.
||Pub. in Jnl. of Pharmacology and Experimental Therapeutics, v263 n3 p1432-1439 Dec 92. Prepared in cooperation with North Carolina State Univ. at Raleigh. Dept. of Toxicology, ManTech Environmental Technology, Inc., Research Triangle Park, NC., and Chemical Industry Inst. of Toxicology, Research Triangle Park, NC.
|NTIS Title Notes
||Reprint: Olfactory Toxicity of Beta,Beta'-Iminodipropionitrile in the Rat.
||PC A02/MF A01