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OLS Field Name OLS Field Data
Main Title Anomalies in the Enumeration of Starved Bacteria on Culture Media Containing Nalidixic Acid and Tetracycline.
Author Genthner, F. J. ; Upadhyay, J. ; Campbell, R. P. ; Genthner, B. R. S. ;
CORP Author Environmental Research Lab., Gulf Breeze, FL. ;Virginia Polytechnic Inst. and State Univ., Blacksburg. ;Technical Resources, Inc., Gulf Breeze, FL.
Publisher c1990
Year Published 1990
Report Number EPA/600/J-90/515;
Stock Number PB91-206870
Additional Subjects Pseudomonas fluorescens ; Culture media ; Tetracycline ; Nalidixic acid ; Antibiotic resistance ; Microbial drug resistance ; Genetic engineering ; Catalase ; Starvation ; Microbial colony count ; Reprints ;
Holdings
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NTIS  PB91-206870 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 11/26/1991
Collation 8p
Abstract Culturable counts of antibiotic resistant, genetically engineered Pseudomonas fluorescens were determined on antibiotic-containing plate count agar during starvation in water. The mean of the colony counts on plate count agar with or without tetracycline (4.9 times ten to the sixth power/ml) was significantly higher than mean colony counts on plate count agar containing either nalidixic acid or nalidixic acid plus tetracycline (2.5 times ten to the sixth power/ml). After 20 days of starvation the highest mean colony counts continued to be obtained on plate count agar (7.2 times ten to the sixth power/ml) with slightly, but significantly, lower counts obtained on plate count agar containing either nalidixic acid (5.6 times ten to the sixth power/ml) or tetracylcline (1.5 times ten to the sixth power/ml). A combination of nalidixic acid and tetracycline in plate count agar, however, dramatically reduced colony counts (8.3 x 100/ml) after the starvation period. The addition of catalase to plate count agar containing nalidixic acid and tetracycline negated the effect caused by the combination.
Supplementary Notes Pub. in Microbial Ecology 20, p283-288 1990. Prepared in cooperation with Virginia Polytechnic Inst. and State Univ., Blacksburg, and Technical Resources, Inc., Gulf Breeze, FL.
NTIS Title Notes Journal article.
Title Annotations Reprint: Anomalies in the Enumeration of Starved Bacteria on Culture Media Containing Nalidixic Acid and Tetracycline.
Category Codes 57K; 57Q; 57F
NTIS Prices PC A02/MF A01
Primary Description 600/04
Document Type NT
Cataloging Source NTIS/MT
Control Number 124123180
Origin NTIS
Type CAT