||2,4,5-Trichlorophenoxyacetic Acid Influence on 2,6-Dinitrotoluene-Induced Urine Genotoxicity in Fisher 344 Rats: Effect on Gastrointestinal Microflora and Enzyme Activity.
George, S. E. ;
Chadwick, R. W. ;
Chang, J. J. ;
Kohan, M. J. ;
Allison, J. C. ;
||Health Effects Research Lab., Research Triangle Park, NC. Neurotoxicology Div. ;North Carolina Univ. at Chapel Hill. ;Environmental Health Research and Testing, Inc., Research Triangle Park, NC.
Gastrointestinal system ;
Dose-response relationships ;
Metabolic activation ;
Trichlorophenoxyacetic acids ;
||Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy.
||2,4,5-Trichlorophenoxyacetic acid and 2,6-dinitrotoluene are hazardous chemicals that have potential harmful effects. 2,6-DNT is recognized as a hepatoxicant while 2,4,5-T, a component in Agent Orange, is also suspect. 2,6-DNT requires both oxidative and reductive metabolism to elicit genotoxic effects. In order to determine what effect 2,4,5-T had on 2,6-DNT metabolism, intestinal enzymes, microbial populations, and urine mutagenicity were examined during 2,4,5-T treatment. Weanling Fischer 344 male rats were treated daily with 54.4 mg/kg 2,4,5-T by gavage for 4 weeks. 1,2, and 4 weeks after the initial dose, rats were administered (p.o.) 2,6-DNT (75 mg/kg) and urine was collected for 24 hours in metabolism cages. Azo reductase, nitroreductase, beta-glucuronidase, dechlorinase, and dehydrochlorinase activities were examined concurrently. Treatment of rats for one week reduced the transformation of 2,6-DNT to mutagenic urinary metabolites. This was accompanied by a decrease in the fecal anaerobic microorganisms. The elimination of Lactobacillus fermentum from the small intestine and cecum of treated animals accompanied a significant increase in oxygen tolerant lactobacilli and other unidentified aerobic microorganisms. (Copyright (c) 1992 by the Society of Toxicology.)
||Pub. in Fundamental and Applied Toxicology, v18 n2 p240-246 Feb 92. Prepared in cooperation with North Carolina Univ. at Chapel Hill, and Environmental Health Research and Testing, Inc., Research Triangle Park, NC.
|NTIS Title Notes
||Reprint: 2,4,5-Trichlorophenoxyacetic Acid Influence on 2,6-Dinitrotoluene-Induced Urine Genotoxicity in Fisher 344 Rats: Effect on Gastrointestinal Microflora and Enzyme Activity.
||57Y; 57F; 57K
||PC A02/MF A01