Main Title |
Flow Cytometric Analysis of the Cellular Toxicity of Tributyltin. |
Author |
Zucker, R. M. ;
Elstein, K. H. ;
Easterling, R. E. ;
Massaro, E. J. ;
|
CORP Author |
Health Effects Research Lab., Research Triangle Park, NC. ;Northrop Services, Inc./Environmental Sciences, Research Triangle Park, NC. |
Publisher |
c1988 |
Year Published |
1988 |
Report Number |
EPA/600/J-88/489; |
Stock Number |
PB90-186263 |
Additional Subjects |
Toxicity ;
Deoxyribonucleic acids ;
Cytoplasm ;
Proteins ;
Cells(Biology) ;
Light scattering ;
Reprints ;
Tributylin ;
Flow cytometry ;
Fluorescence microscopy ;
Dose-response relationships
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
NTIS |
PB90-186263 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Collation |
20p |
Abstract |
Flow cytometric and light/fluorescence microscopic analyses indicate that tributylin (TBT) alters the plasma membrane/cytoplasm complex of the murine erythroleukemic cell (MELC) in a dose-dependent and time-dependent manner. The flow cytometric parameter axial light loss, a measure of cell volume, decrease cell exposed to 5 mu M TBT relative to control cells or cells exposed to 50 mu M TBT. The flow cytometric parameter 90 degree light scatter, a function of refractive index and a measure of protein content, increases as a function of TBT concentration above 0.5 mu M. Following exposure to TBT concentrations greater than 0.5 mu M but less than 50 mu M, DNA distribution across the cell cycle cannot be resolved adequately by flow cytometry. Also, the cells become resistant to solubilization of the cell membrane/cytoplasm complex by nonionic detergents. |
Supplementary Notes |
Pub. in Toxicology Letters, v43 p201-218 Sep 88. Prepared in cooperation with Northrop Services, Inc./Environmental Sciences, Research Triangle Park, NC. |
NTIS Title Notes |
Journal article. |
Title Annotations |
Reprint: Flow Cytometric Analysis of the Cellular Toxicity of Tributyltin. |
Category Codes |
57Y; 57F |
NTIS Prices |
PC A03/MF A01 |
Primary Description |
600/10 |
Document Type |
NT |
Cataloging Source |
NTIS/MT |
Control Number |
011722497 |
Origin |
NTIS |
Type |
CAT |