||Optimization of the Hamilton-Thorn Computerized Sperm Motility Analysis System for Use with Rat Spermatozoa in Toxicological Studies.
Slott, V. L. ;
Suarez, J. D. ;
Poss, P. M. ;
Linder, R. E. ;
Strader, L. F. ;
||ManTech Environmental Technology, Inc., Research Triangle Park, NC.;Health Effects Research Lab., Research Triangle Park, NC. Reproductive Toxicology Branch.
Sperm motility ;
Computer simulation ;
Reproducibility of results ;
Risk assessment ;
||Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy.
||To optimize the Hamilton-Thorn Motility Analyzer (HTM; Hamilton-Thorn Research, Beverly, MA) for use in reproductive toxicology studies with rat spermatozoa, the accuracy and precision of the instrument were assessed under a variety of instrument settings. Videotapes of both fast- and slow-swimming sperm were analyzed repeatedly to obtain data across a range of sperm velocities as might be encountered as a consequence of exposure to reproductive toxicants. Acquisition rates were varied across the HTM menu choices as were the number of frames analyzed at each framing rate. For fast-swimming samples (mean straight-line velocity (VSL) approximately 130 micrometers/sec) generally good agreement between computer-assisted sperm analysis (CASA) and manually obtained data was found for percentage of motile sperm and straight-line velocity; i.e., CASA values were within 10% of manual values for most frame/rate combinations. (Copyright (c) 1993 by the Society of Toxicology.)
||Pub. in Fundamental and Applied Toxicology, v21 n3 p298-307 Oct 93. Sponsored by Health Effects Research Lab., Research Triangle Park, NC. Reproductive Toxicology Branch.
|NTIS Title Notes
||Reprint: Optimization of the Hamilton-Thorn Computerized Sperm Motility Analysis System for Use with Rat Spermatozoa in Toxicological Studies.
||PC A03/MF A01