| Main Title |
Bioassay-Directed Fractionation of Organic Contaminants in an Estuarine Sediment Using the New Mutagenic Bioassay, Mutatox (Trade Name). |
| Author |
Ho, K. T. Y. ;
Quinn, J. G. ;
|
| CORP Author |
Environmental Research Lab., Narragansett, RI. ;Rhode Island Univ., Kingston. Graduate School of Oceanography. |
| Publisher |
c1993 |
| Year Published |
1993 |
| Report Number |
EPA/600/J-93/183 ;ERLN-1438; |
| Stock Number |
PB93-194504 |
| Additional Subjects |
Bioassay ;
Fractionation ;
Organic compounds ;
Sediments ;
Water pollution detection ;
Mutagens ;
Toxicity ;
Connecticut ;
Chromatographic analysis ;
Mass spectroscopy ;
Chemical analysis ;
Reprints ;
Black Rock Harbor(Connecticut)
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB93-194504 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
10p |
| Abstract |
Bioassay-directed fractionation of organic compounds was performed on an organic solvent extract of a contaminated estuarine sediment from Black Rock Harbor, Connecticut, using the new mutagenic bioassay, Mutatox. Chemical fractionation methods of the sediment extract included silica-gel-column chromatography (SGCC), followed by C-18 reverse phase HPLC. Compound identification was performed using GC-MS. Mutatox analyses indicated that four of the eight HPLC fractions contained mutagenic or epigenetic compounds. GC-MS analyses detected known mutagenic polycyclic aromatic hydrocarbons (PAHs) in two of the four mutagenic fractions. GC-MS analyses of the other two mutagenic fractions (one operational blank and one sediment fraction) showed no known mutagenic compounds. SGCC and HPLC, combined with the Mutatox bioassay, were useful in reducing both the number and the complexity of fractions analyzed by GC-MS. (Copyright (c) 1993 SETAC.) |
| Supplementary Notes |
Pub. in Environmental Toxicology and Chemistry, v12 p823-830 1993. Prepared in cooperation with Rhode Island Univ., Kingston. Graduate School of Oceanography. |
| NTIS Title Notes |
Journal article. |
| Title Annotations |
Reprint: Bioassay-Directed Fractionation of Organic Contaminants in an Estuarine Sediment Using the New Mutagenic Bioassay, Mutatox (Trade Name). |
| Category Codes |
68D; 57Y; 99A |
| NTIS Prices |
PC A02/MF A01 |
| Primary Description |
600/05 |
| Document Type |
NT |
| Cataloging Source |
NTIS/MT |
| Control Number |
320426589 |
| Origin |
NTIS |
| Type |
CAT |
