Main Title |
Circulating Factors that Modify Lung Cell DNA Synthesis Following Exposure to Inhaled Oxidants. 1. Effect of Serum and Lavage on Lung Fibroblasts Following Exposure of Adult Rats to 1 ppm Ozone. |
Author |
Tanswell, A. K. ;
Fraher, L. J. ;
Grose, E. C. ;
|
CORP Author |
Health Effects Research Lab., Research Triangle Park, NC. ;University of Western Ontario, London. |
Publisher |
c1989 |
Year Published |
1989 |
Report Number |
EPA/600/J-89/216; |
Stock Number |
PB90-146143 |
Additional Subjects |
Deoxyribonucleic acids ;
Lung ;
Ozone ;
Respiration ;
Exposure ;
Blood ;
Cells(Biology) ;
Oxidizers ;
pH ;
Bioassay ;
Statistical analysis ;
Reprints ;
Bronchoalveolar lavage fluid ;
Fibroblasts ;
Isoelectric focusing ;
High performance liquid chromatography
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
NTIS |
PB90-146143 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Collation |
18p |
Abstract |
Adult rats were exposed to 1 ppm (1.96 mg/cu m) ozone or air for 2 weeks. Animals were sacrificed at 3, 5, 7, or 14 days after the onset of exposure and samples of plasma and lung lavage obtained. Heat inactivated plasma, from animals exposed to ozone for 7 or 14 days, significantly increased DNA synthesis by lung fibroblasts compared with plasma from air exposed animals. Fractionation of plasma and lavage samples indicated that the factor responsible had an isoelectric point of 6.45-6.75, and a molecular weight of 32 +/- 2 Kd. This factor has a dose-dependent effect on lung fibroblasts in culture, but no significant effect on cultured pneumocyte DNA synthesis. The factor is detectable within 72 hours of exposure, and may hold some promise as a marker of early oxidant lung injury. (Copyright (c) 1989, Hemisphere Publishing Corporation.) |
Supplementary Notes |
Pub. in Jnl. of Toxicology and Environmental Health 27, p239-254 1989. Prepared in cooperation with University of Western Ontario, London. |
NTIS Title Notes |
Journal article. |
Title Annotations |
Reprint: Circulating Factors that Modify Lung Cell DNA Synthesis Following Exposure to Inhaled Oxidants. 1. Effect of Serum and Lavage on Lung Fibroblasts Following Exposure of Adult Rats to 1 ppm Ozone. |
Category Codes |
57Y; 57F |
NTIS Prices |
PC A03/MF A01 |
Primary Description |
600/10 |
Document Type |
NT |
Cataloging Source |
NTIS/MT |
Control Number |
007530433 |
Origin |
NTIS |
Type |
CAT |