||Formation of Mutagenic Urinary Metabolites from 1-Nitropyrene in Germ-Free and Conventional Rats: Role of the Gut Flora.
Ball, L. M. ;
Rafter, J. J. ;
Gustafsson, J. A. ;
Gustafsson, B. E. ;
Kohan, M. J. ;
||Health Effects Research Lab., Research Triangle Park, NC. Genetic Toxicology Div. ;Karolinska Inst., Stockholm (Sweden).
Mutagenicity tests ;
Liquid chromatography ;
Environmental pollutants ;
Metabolic activation ;
||Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy.
||1-Nitropyrene (NP), an environmental pollutant, a potent mutagen and an animal carcinogen, undergoes reduction, acetylation, ring-hydroxylation and conjugation in the rat in vivo to form mutagenic metabolities which are excreted in the urine. In order to investigate the role of the gut flora in the generation of these metabolities, germ-free rats of the AGUS strain, and conventional AGUS rats matched for sex and age, where injected intraperitoneally with NP labelled with (14)C. When urines were examined for mutagenicity with the Ames plate incorporation assay, the highest mutagenic activity was seen in 8-24 h urine from conventional rats in the presence of S9. These results indicate that the gut flora are necessarity involved in the formation of 6-hydroxy-1-acetamidopyrene, and their role may include both introreduction and the hydrolysis of glucuronides released from enterohepatic recirculation.
||Pub. in Carcinogenesis, v12 n1 p1-5 1991. Prepared in cooperation with Karolinska Inst., Stockholm (Sweden).
|NTIS Title Notes
||Reprint: Formation of Mutagenic Urinary Metabolites from 1-Nitropyrene in Germ-Free and Conventional Rats: Role of the Gut Flora.
||57Y; 57F; 57K; 68
||PC A02/MF A01