||Acute Exposure of the Neonatal Rat to Triethyltin Results in Persistent Changes in Neurotypic and Gliotypic Proteins (Journal Version).
O'Callaghan, J. P. ;
Miller, D. B. ;
||Health Effects Research Lab., Research Triangle Park, NC.
||22 Sep 87
Nerve cells ;
Laboratory animals ;
Tin organic compounds ;
Triethyl tin ;
Nerve tissue proteins ;
||Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy.
||Measurements of neuron-specific (neurotypic) and glia-specific (fliotypic) proteins were used to characterize the toxic effects of TET on the developing CNS. Six proteins, each of which is associated with specific aspects of neuronal and glial development, were evaluated as follows: (1) NF-200, an intermediate filament protein of the neuronal cytoskeleton; (2) synapsin I, a synapse-specific, synaptic vesicle localized protein;(3) p38, another synaptic-vesicle localized protein; (4) MBP, a protein unique to myelin-forming oligodendroglia; (5) GFAP, the intermediate filament protein of astrocytes; and (6) beta-tubulin, a constituent primarily of neuronal microtubules. The amount of each protein in homogenates of hippocampus, forebrain and cerebellum, brain regions with different developmental profiles, was determined by RIA. TET (3 or 6 mg/kg, i.p.) on postnatal day 5 caused permanent dose- and region-dependent decrements in brain weight, with the hippocampus being the most affected. The effects were not associated with light microscopic evidence of cytopathology but were accompanied by large dose-, time- and region-dependent alterations in all neurotypic and gliotypic proteins evaluated.
||Pub. in Jnl. of Pharmacology and Experimental Therapeutics, v244 n1 p368-378 1988.
|NTIS Title Notes
||Reprint: Acute Exposure of the Neonatal Rat to Triethyltin Results in Persistent Changes in Neurotypic and Gliotypic Proteins (Journal Version).
||PC A03/MF A01