Main Title |
Gene Probes as a Tool for the Detection of Specific Genomes in the Environment. |
Author |
Barkay, T. ;
Sayler, G. ;
|
CORP Author |
Environmental Research Lab., Gulf Breeze, FL. ;Tennessee Univ., Knoxville. |
Year Published |
1986 |
Report Number |
EPA/600/D-86/215; |
Stock Number |
PB87-102505 |
Additional Subjects |
Molecular biology ;
Detection ;
Sensitivity ;
Specificity ;
Gene probes
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
NTIS |
PB87-102505 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Collation |
22p |
Abstract |
Gene probes hold a great promise as a tool in environmental sciences. They may be used to detect specific genotypes, to follow gene flow process, to delineate complex taxonomic aggregates and to monitor genetically engineered organisms in the environment. The sensitivity of the method is currently limited by experimental procedures and its specificity depends on the nature of the DNA sequences used as probes and the efficacy of lysing methods. Variable genetic determinants which code for the same trait determine the universality of gene probes. Finally, the method is highly feasible in terms of cost, speed and expertise. Current and future developments in molecular microbial ecology are likely to contribute toward the improvement of the probing methodology for the full realization of its potential in environmental sciences. |
Supplementary Notes |
Prepared in cooperation with Tennessee Univ., Knoxville. |
PUB Date Free Form |
Sep 86 |
Category Codes |
57F |
NTIS Prices |
PC A02/MF A01 |
Primary Description |
600/04 |
Document Type |
NT |
Cataloging Source |
NTIS/MT |
Control Number |
633717155 |
Origin |
NTIS |
Type |
CAT |