||Gene Structure in Methanogenic Bacteria.
Reeve, J. N. ;
||Ohio State Univ., Columbus. Dept. of Microbiology.;Environmental Protection Agency, Cincinnati, OH. Risk Reduction Engineering Lab.;Department of Energy, Washington, DC.
||EPA-R-810340, DE-AC02-81ER10945; EPA/600/J-87/495;
Deoxyribonucleic acids ;
Escherichia coli ;
Bacillus subtilis ;
Bacterial genes ;
Genetic translation ;
Molecular cloning ;
Base sequence ;
Nucleic acid hybridization
||Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy.
||Methane-producing microorganisms (methanogens) catalyze the terminal reactions in the anaerobic digestion of biomass. This is a well-established biotechnological process used for both waste treatment and the production of the renewal energy source methane. Although methanogens are procaryotes they are not closely related to well-known eubacterial species, commonly termed bacteria, but are members of the third biological kingdom, the Archaebacteria. The paper presents a brief review of the information currently available documenting the structure of genes in methanogens. A comparison of gene structure in eubacteria, eucaryotes and methanogens is given. Evidence is presented indicating that polypeptide-encoding genes in methanogens are organized into operons and that translation is coordinated, as in eubacteria, by messenger ribonucleic acid:16S ribosomal ribonucleic acid hybridization. Methanogen-derived genes are expressed when cloned in Escherichia coli, which should allow routine genetic engineering procedures to be used to dissect and manipulate the bioprocess of methanogenesis.
||Pub. in Poultry Science 66, p927-933 1987. Sponsored by Environmental Protection Agency, Cincinnati, OH. Risk Reduction Engineering Lab., and Department of Energy, Washington, DC.
|NTIS Title Notes
||57F; 57K; 68G
||PC A02/MF A01