Full Record Display for the EPA National Library Catalog

RECORD NUMBER: 3 OF 8

OLS Field Name OLS Field Data
Main Title Disruption of the 'Saccharomyces cerevisiae' Gene for NADPH-Cytochrome P450 Reductase Causes Increased Sensitivity to Ketoconazole.
Author Sutter, T. R. ; Loper, J. C. ;
CORP Author Cincinnati Univ., OH. Coll. of Medicine.;Environmental Protection Agency, Cincinnati, OH. Risk Reduction Engineering Lab.
Publisher c1989
Year Published 1989
Report Number EPA-CR-813366; EPA/600/J-93/305;
Stock Number PB93-229276
Additional Subjects Fungal genes ; NADH dehydrogenase ; Saccharomyces cerevisiae ; Ketoconazole ; Microbial sensitivity tests ; Plasmids ; Genetic complementation tests ; Southern blotting ; Western blotting ; Reprints ; Phenotype ; Candida tropicalis
Holdings
Library Call Number Additional Info Location Last
Modified
Checkout
Status
NTIS  PB93-229276 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 11/22/1993
Collation 12p
Abstract Strains of Saccharomyces cerevisiae deleted in the NADPH-cytochrome P450 reductase gene by transplacement are 200-fold more sensitive to ketoconazole, an inhibitor of the cytochrome P450 lanosterol 14alpha-demethylase. Resistance is restored through complementation by the plasmid-borne wild type gene from either S. cerevisiae or Candida tropicalis. Neither Southern hybridization nor Western immunoblot techniques provided evidence for a second NADPH-cytochrome P450 reductase gene, suggesting that an alternate pathway may provide for the functions of this reductase in S. cerevisiae. (Copyright (c) 1989 Academic Press, Inc.)
Supplementary Notes Pub. in Biochemical and Biophysical Research Communications, v160 n3 p1257-1266 May 89. Sponsored by Environmental Protection Agency, Cincinnati, OH. Risk Reduction Engineering Lab.
NTIS Title Notes Journal article.
Title Annotations Reprint: Disruption of the 'Saccharomyces cerevisiae' Gene for NADPH-Cytochrome P450 Reductase Causes Increased Sensitivity to Ketoconazole.
Category Codes 57F; 57K; 57Q
NTIS Prices PC A03/MF A01
Primary Description 600/14
Document Type NT
Cataloging Source NTIS/MT
Control Number 328023357
Origin NTIS
Type CAT