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RECORD NUMBER: 279 OF 1236

OLS Field Name OLS Field Data
Main Title Encystation and Expression of Cyst Antigens by 'Giardia lamblia' In vitro.
Author Gillin, F. D. ; Reiner, D. S. ; Gault, M. J. ; Douglas, H. ; Das, S. ;
CORP Author California Univ. Medical Center, San Diego.;Health Effects Research Lab., Cincinnati, OH.
Year Published 1987
Report Number EPA/600/J-87/021;
Stock Number PB87-196150
Additional Subjects Giardia ; Cysts ; Antigens ; Giardiasis ; Gastrointestinal diseases ; In vitro analysis ; Immunology ; Morphology ; Laboratory animals ; Mice ; Reprints ; Encystation
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NTIS  PB87-196150 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 06/21/1988
Collation 6p
Abstract The cyst form of Giardia lamblia is responsible for transmission of giardiasis, a major waterborne intestinal disease. These studies demonstrate for the first time expression of cyst antigens and encystation of G. lamblia in vitro by both morphologic and immunologic criteria. The researchers first showed that the jejunum is a major site of encystation in the suckling mouse model and therefore tested small intestinal factors as stimuli of encystation. As a sensitive probe for differentiation in vitro, researchers raised antisera which react with cysts and precysts, but not with cultured trophozoites. Exposure of cultured trophozoites to glycodeoxycholate, a bile salt, increased the number of oval, refractile cells which react strongly with anticyst antibodies from <0.1% to 8.9%. Encystation expression under anaerobic conditions reached 51.2%. In vitro expression of a major cyst antigen of approximately 26,000 daltons (26K) was demonstrated on immunoblots. These studies are an important step in understanding the differentiation of a major protozoan pathogen. (Copyright (c) 1987, Science.)
Supplementary Notes Pub. in Science, v235 p1040-1043 Feb 87. Sponsored by Health Effects Research Lab., Cincinnati, OH.
NTIS Title Notes Journal article.
Title Annotations Reprint: Encystation and Expression of Cyst Antigens by 'Giardia lamblia' In vitro.
PUB Date Free Form c1987
Category Codes 57N; 57J; 57K; 57Y; 68G
NTIS Prices PC A02/MF A01
Primary Description 600/11
Document Type NT
Cataloging Source NTIS/MT
Control Number 723225505
Origin NTIS
Type CAT