||Comparison of In vivo and In vitro Methods for Assessing the Effects of Carbon Tetrachloride on the Hepatic Drug-Metabolizing Enzyme System.
Chadwick, R. W. ;
Copeland, M. F. ;
Carlson, G. P. ;
Trela, B. A. ;
Most, B. M. ;
||Health Effects Research Lab., Research Triangle Park, NC. ;Northrop Services, Inc., Research Triangle Park, NC. ;Purdue Univ., Lafayette, IN. Dept. of Pharmacology and Toxicology.
Carbon tetrachloride ;
In vitro analysis ;
In vivo analysis ;
UDP glucuronosyltransferase ;
Glutathione transferases ;
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||The effect of a single i.p. injection of 0, 20, 200, and 1000 microliters/kg carbon tetrachloride on the activity of the hepatic drug-metabolizing enzyme system was measured in the rat by a model substrate assay, employing lindane (gamma-hexachlorocyclohexane), and by a battery of in vitro enzyme assays. The data in the study indicated that carbon tetrachloride had a biphasic influence on the phase I reactions with the lowest dose inducing a significant increase in enzyme activity while the highest dose produced significant inhibition. Significant CCl4-induced reductions in glucuronyltransferase and sulfotransferase activities were also observed while the effect on glutathione-S-transferase was ambiguous. The in vivo and in vitro assays showed good agreement.
||Pub. in Toxicology Letters, v42 n3 p309-316 Sep 88. Prepared in cooperation with Northrop Services, Inc., Research Triangle Park, NC., and Purdue Univ., Lafayette, IN. Dept. of Pharmacology and Toxicology.
|NTIS Title Notes
||Reprint: Comparison of In vivo and In vitro Methods for Assessing the Effects of Carbon Tetrachloride on the Hepatic Drug-Metabolizing Enzyme System.
||PC A02/MF A01