||Detection of O6-Methyldeoxyguanosine in Human Placental DNA.
Foiles, P. G. ;
Miglietta, L. M. ;
Akerkar, S. A. ;
Everson, R. B. ;
Hecht, S. S. ;
||American Health Foundation, Inc., Valhalla, NY. Naylor Dana Inst. for Disease Prevention. ;National Inst. of Environmental Health Sciences, Research Triangle Park, NC. Epidemiology Branch.;Health Effects Research Lab., Research Triangle Park, NC.;National Cancer Inst., Bethesda, MD.
Deoxyribonucleic acids ;
Monoclonal antibodies ;
Antibody binding sites ;
High pressure liquid chromatography
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||A monoclonal antibody specific for O(sup 6)-methyldeoxyguanosine (O(sup 6)-MedGuo) was developed. When used in a competitive ELISA, 50% inhibition of binding was achieved with 0.51 pmol O(sup 6)-MedGuo. When the competitive ELISA was coupled with HPLC, 2 mg of DNA could be analyzed giving a lower limit of detection of 0.5 micromol O(sup 6)-MedGuo per mol deoxyguanosine. The assay was used to test for O(sup 6)-MedGuo in DNA from placentas of smoking and non-smoking women. Two of 10 DNA samples from smoking women and 3 of 10 from non-smoking women had detectable levels of O(sup 6)-MedGuo. Levels ranged from 0.6 to 1.6 micromol O(sup 6)-MedGuo per mol deoxyguanosine. Levels of O(sup 6)-alkylguanine DNA alkyltransferase (AAT) protein were also measured. There was no apparent relationship between AAT and O(sup 6)-MedGuo levels in the 20 subjects, nor did mean AAT levels differ between the 2 groups. Although no apparent relationship between smoking history and O(sup 6)-MedGuo formation was found in the preliminary study, the report is the first of a structurally identified DNA adduct in human placenta.
||Pub. in Cancer Research, v48 p4184-4188 Aug 88. Prepared in cooperation with National Inst. of Environmental Health Sciences, Research Triangle Park, NC. Epidemiology Branch. Sponsored by Health Effects Research Lab., Research Triangle Park, NC., and National Cancer Inst., Bethesda, MD.
|NTIS Title Notes
||Reprint: Detection of O6-Methyldeoxyguanosine in Human Placental DNA.
||PC A02/MF A01