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RECORD NUMBER: 493 OF 1079

Main Title Microbial Degradation of 2,4,5-T and Chlorinated Dioxins.
Author Chakrabarty, A. M. ;
CORP Author Illinois Univ. at the Medical Center, Chicago.;Environmental Protection Agency, Cincinnati, OH. Hazardous Waste Engineering Research Lab.
Year Published 1987
Report Number EPA-R-809666; EPA/600/M-86/025;
Stock Number PB87-153789
Additional Subjects Soils ; Decontamination ; Biodeterioration ; Chlorine organic compounds ; Microorganisms ; Herbicides ; Chlorine aromatic compounds ; Microbial degradation ; T 2-4-5 herbicide ; Pseudomonas cepacia ; Acetic acid/trichlorophenoxy
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https://nepis.epa.gov/Exe/ZyPDF.cgi?Dockey=300024FD.PDF
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NTIS  PB87-153789 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 4p
Abstract The report from the University of Illinois College of Medicine at Chicago covers three years of research under a Cooperative Agreement. A pure culture of Pseudomonas capacia strain AC1100, isolated from a chemostat enrichment culture experiment, is capable of growing on 2,4,5-T as its sole source of carbon and energy. Metabolic pathway studies indicate the activity of both constitutive and inducible enzymes. In laboratory experiments, soil contaminated with 2,4,5-T could be detoxified by AC100 treatment, with the titer of AC1100 rapidly falling to nearly undetectable levels after the 2,4,5-T was substantially degraded. Extensive homology observed between plasmids points to the role of plasmid genes in the evolution and spread of degradative characters against toxic chemical compounds. A 1.3 kilobase pair DNA repeated sequence was found in strain AC1100. The specificity of this sequence to AC1100 suggests that this unique sequence may be a useful genetic probe of strain AC1100 and other novel Pseudomonas strains which are under consideration for deliberate release to aquatic and terrestrial environments.
Supplementary Notes Sponsored by Environmental Protection Agency, Cincinnati, OH. Hazardous Waste Engineering Research Lab.
NTIS Title Notes Environmental research brief.
PUB Date Free Form Jan 87
Category Codes 68C; 57M
NTIS Prices PC A02/MF A01
Primary Description 600/12
Document Type NT
Cataloging Source NTIS/MT
Control Number 712831498
Origin NTIS
Type CAT