| Main Title |
In Vitro Gene Mutation Test with Isobutyraldehyde in Cho Cells (HPRT Locus Assay), with Cover Letter dated 08/03/1999. |
| CORP Author |
BASF Corp., Mount Olive, NJ.; BASF A.G., Ludwigshafen am Rhein (Germany, F.R.). Aktiengesellschaft labor fuer Umweltanalytik und Okologie.; Environmental Protection Agency, Washington, DC. Office of Toxic Substances. |
| Year Published |
1999 |
| Stock Number |
OTS0573897 |
| Additional Subjects |
Toxicology ;
Health effects ;
Isobutyraldehyde ;
Genotoxicity ;
Chromosomal effects ;
Mammals ;
Hamsters ;
In vitro ;
CAS No 78-84-2
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
OTS0573897 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
65p |
| Abstract |
The CHO/HPRT test detects forward mutations of the X-linked hypoxanthine-guanine phosphoribosyl transferase (HGPRT) locus in Chinese hamster ovary (CHO) cells (1, 2). HPRT catalyzes not only the conversion of hypoxanthine or guanine to the corresponding nucleotides (so-called salvage pathway) but also the conversion of, e.g., the nontoxic purine analogue 6-thioguanine (6TG) to its ribophosphorylated derivative, which is then lethal to the cells. Therefore, a loss of the enzyme or its activity leads to resistance to 6TG, i.e. cells are unable to form lethal nucleotides and thus will grow and form colonies in the presence of the purine analogue. Most of the substances are not mutagenic or carcinogenic themselves, but only after metabolic transformation, which in vivo is catalyzed mainly by the enzyme systems of the liver. Therefore, the tests are carried out not only directly, but also in the presence of an exogenous metabolic activation system. The most commonly used system is a cofactor- supplemented postmitochondrial fraction (S-9) obtained from livers of rats treated with an enzyme-inducing agent, e.g. Aroclor 1254. The aim of the present study was to assess the potential of the test substance Isobutyraldehyde or its metabolite(s) to induce gene mutations in CHO cells. The study was initiated on February 22, 1999 and the practical work was completed on March 17, 1999 (1st experiment) and on May 18, 1999 (2nd experiment). |
| Supplementary Notes |
The quality of the documents listed in the Office of Toxic Substances database may not meet usual NTIS standards but are included to further the opportunity for the scientific and technical community to locate materials which may not be available elsewhere. The content is the responsibility of OTS. Sponsored by Environmental Protection Agency, Washington, DC. Office of Toxic Substances. |
| Availability Notes |
Product reproduced from digital image. Order this product from NTIS by: phone at 1-800-553-NTIS (U.S. customers); (703)605-6000 (other countries); fax at (703)605-6900; and email at orders@ntis.gov. NTIS is located at 5285 Port Royal Road, Springfield, VA, 22161, USA. |
| PUB Date Free Form |
9 Aug 1999 |
| Category Codes |
57Y; 57U; 68G; 99; 44G |
| NTIS Prices |
PC A05/MF A05 |
| Document Type |
NT |
| Cataloging Source |
NTIS/MT |
| Control Number |
028701990 |
| Origin |
NTIS |
| Type |
CAT |
