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OLS Field Name OLS Field Data
Main Title 'Giardia muris': Scanning Electron Microscopy of In vitro Excystation.
Author Coggins, J. R. ; Schaefer,III, F. W. ;
CORP Author Health Effects Research Lab., Cincinnati, OH. ;Wisconsin Univ.-Milwaukee. Dept. of Zoology.
Year Published 1984
Report Number EPA/600/J-84-081;
Stock Number PB84-244094
Additional Subjects Electron microscopy ; Parasites ; In vitro analysis ; Mice ; Cultivation ; Reprints ; Giardia muris ; Excystation
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NTIS  PB84-244094 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 06/23/1988
Collation 10p
Abstract A recently developed in vitro excystation procedure results in almost total excystation of Giardia muris, an intestinal parasite of mice. The present experiment examines the G. muris cyst morphology by scanning electron microscopy and evaluates the efficacy of the excystation procedure. Untreated cysts of G. muris were elliptical and displayed a distinctive surface structure. Excystation began almost immediately after incubation had begun and most trophozoites emerged within 30 minutes. Excystation appears to involve flagellar action of the encysted trophozoite. A tear of the wall occurred at one pole. This opening was subsequently enlarged, presumably by flagellar action. Trophozoites emerged, posterior end first, and an associated mucoid-like material was extruded. Newly emerged trophozoites were nearly oval in shape. Trophozoites quickly became flattened, elongated and underwent cytokinesis resulting in two daughter trophozoites. Few non-excysted organisms were seen after 30 minutes incubation.
Supplementary Notes Prepared in cooperation with Wisconsin Univ.-Milwaukee. Dept. of Zoology.
Availability Notes Pub. in Experimental Parasitology 57, p62-67 1984.
NTIS Title Notes Journal article.
Title Annotations Reprint: 'Giardia muris': Scanning Electron Microscopy of In vitro Excystation.
PUB Date Free Form c1984
Category Codes 6C; 57N
NTIS Prices Not available NTIS
Primary Description 600/11
Document Type NT
Cataloging Source NTIS/MT
Control Number 435625593
Origin NTIS
Type CAT