||Flow Cytometric Analysis of the Cellular Toxicity of Tributyltin.
Zucker, R. M. ;
Elstein, K. H. ;
Easterling, R. E. ;
Massaro, E. J. ;
||Health Effects Research Lab., Research Triangle Park, NC. ;Northrop Services, Inc./Environmental Sciences, Research Triangle Park, NC.
Deoxyribonucleic acids ;
Light scattering ;
Flow cytometry ;
Fluorescence microscopy ;
||Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy.
||Flow cytometric and light/fluorescence microscopic analyses indicate that tributylin (TBT) alters the plasma membrane/cytoplasm complex of the murine erythroleukemic cell (MELC) in a dose-dependent and time-dependent manner. The flow cytometric parameter axial light loss, a measure of cell volume, decrease cell exposed to 5 mu M TBT relative to control cells or cells exposed to 50 mu M TBT. The flow cytometric parameter 90 degree light scatter, a function of refractive index and a measure of protein content, increases as a function of TBT concentration above 0.5 mu M. Following exposure to TBT concentrations greater than 0.5 mu M but less than 50 mu M, DNA distribution across the cell cycle cannot be resolved adequately by flow cytometry. Also, the cells become resistant to solubilization of the cell membrane/cytoplasm complex by nonionic detergents.
||Pub. in Toxicology Letters, v43 p201-218 Sep 88. Prepared in cooperation with Northrop Services, Inc./Environmental Sciences, Research Triangle Park, NC.
|NTIS Title Notes
||Reprint: Flow Cytometric Analysis of the Cellular Toxicity of Tributyltin.
||PC A03/MF A01