||Formation of DNA Adducts in 1OT1/2 Mouse Embryo Fibroblasts Incubated with Benzo(a)pyrene or Dihydrodiol Oxide Derivatives.
Brown, Halina Szejnwald ;
Jeffrey, Alan M. ;
Weinstein, I. Bernard ;
||Columbia Univ., New York.;Health Effects Research Lab., Research Triangle Park, NC.;National Cancer Inst., Bethesda, MD.
||EPA-R-805482 ;PHS-CA-13696; EPA-600/J-79-148;
Deoxyribonucleic acid ;
Aromatic polycyclic hydrocarbons ;
Oxygen organic compounds ;
Dihydrodiol oxide ;
Cell lines ;
||Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy.
||Binding of benzo(a)pyrene (BP) metabolites to DNA was studied in the mouse embryo fibroblast 10T one half cell line. The cells metabolized BP to its diastereoisomeric 7,8-dihydrodiol-9,10-oxides, (+)-7B, 8a-dihydroxy-9B, 10B-epoxy-7,8,9,10-tetrahydrobenzo(a)pyrene, which formed covalent adducts with cellular DNA. Detailed analysis by high-pressure liquid chromatography indicated that a deoxyguanosine adduct, N2-(10S-(7R, 8S, 9R-trihydroxy-7,8,9,10-tetrahydrobenzo(a)pyrene)yldeoxyguaninosine, was the predominant DNA adduct formed in cells exposed to BP. This adduct is also the major one found in human tissues exposed to BP.
||Pub. in Cancer Research 39, p1673-1677 May 79.
|NTIS Title Notes
||Reprint: Formation of DNA Adducts in 1OT1/2 Mouse Embryo Fibroblasts Incubated with Benzo(a)pyrene or Dihydrodiol Oxide Derivatives.
|PUB Date Free Form
||30 Jan 79
||PC A02/MF A01