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OLS Field Name OLS Field Data
Main Title Method 1681: Fecal Coliforms in Sewage Sludge (Biosolids) by Multiple-Tube Fermentation using A-1 Medium, July 2006.
CORP Author Environmental Protection Agency, Washington, DC. Office of Water.
Publisher Jul 2006
Year Published 2006
Report Number EPA/821/R-06/013;
Stock Number PB2007-101971
Additional Subjects Sewage sludge ; Fermentation ; Detection ; Enumeration ; Coliforms ; Bacteria ; Multiple-tube fermentation ; Fecal coliforms ; A-1 medium ; Biosolids ; Most probable number (MPN) ; EPA method 1681
Library Call Number Additional Info Location Last
NTIS  PB2007-101971 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 05/28/2007
Collation 51p
Abstract This method describes multiple-tube fermentation procedures (also called the most probable number (MPN) procedure) for the detection and enumeration of fecal coliform bacteria in biosolids. These methods use culture-specific media and elevated temperature to isolate and enumerate fecal coliform organisms. Fecal coliform bacteria, including Escherichia coli (E. coli), are commonly found in the feces of humans and other warm-blooded animals, and indicate the potential presence of other bacterial and viral pathogens. This method is adapted from method 9221E in Standard Methods for the Examination of Water and Wastewater, 20th Edition, for the determination of fecal coliform bacteria in a variety of matrices (Reference 19.1). This method is designed to meet the survey and monitoring requirements of the U.S. Environmental Protection Agency (EPA) in regulating the use and disposal of biosolids under 40 CFR Part 503. Subpart D of the 503 regulations protects public health and the environment through requirements designed to reduce the potential for contact with disease-bearing microorganisms (pathogens) in biosolids applied to land or placed on a surface disposal site.
Supplementary Notes See also PB2005-110690.
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Category Codes 57K; 68C
NTIS Prices PC A05/MF A01
Document Type NT
Cataloging Source NTIS/MT
Control Number 005101000
Origin NTIS
Type CAT