Main Title |
Methods for the Detection of Changes in the Microbial Ecology of Soil Caused by the Introduction of Microorganisms. |
Author |
Doyle, J. D. ;
Stotzky, G. ;
|
CORP Author |
New York Univ., NY. Dept. of Biology. ;ManTech Environmental Technology, Inc., Corvallis, OR.;Corvallis Environmental Research Lab., OR. |
Publisher |
1993 |
Year Published |
1993 |
Report Number |
EPA-R813650 ;EPA-R-813431; EPA/600/J-93/502; |
Stock Number |
PB94-136157 |
Additional Subjects |
Soil microbiology ;
Ecology ;
Microorganisms ;
Respiration ;
Carbon dioxide ;
Enzymes ;
Genetic engineering ;
Escherichia coli ;
Species diversity ;
Antiobiotics ;
Measurement ;
Changes ;
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
NTIS |
PB94-136157 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Collation |
12p |
Abstract |
Established methods for measuring a broad spectrum of microbial activities were evaluated for their ability to detect perturbations of the soil environment by introduced microorganisms. These methods included: gross metabolic activity and carbon mineralization, as measured by respiration (CO2 evolution); activity of selected enzymes (arylsulfatases, acid and alkaline phosphatases, dehydrogenases); and indicators of the community structure of the indigenous microbiota (species diversity, physiological groups, antibiotic-resistance profiles). The evaluation of these variables, as well as the fate of the introduced microorganisms (Escherichia coli W3110 (R702) and its homologous, plasmidless, parental strain, W3110), was conducted over a 33-day period. Statistically significant differences only in some components of the microbiota were detected between soil inoculated with W3110 (R702) or W3110 of not inoculated. However, these differences were transient and, therefore, probably not ecologically significant. |
Supplementary Notes |
Pub. in Microbe Releases 2, p63-72 1993. See also PB91-219022. Prepared in cooperation with ManTech Environmental Technology, Inc., Corvallis, OR. Sponsored by Corvallis Environmental Research Lab., OR. |
NTIS Title Notes |
Journal article. |
Category Codes |
48E; 57K; 57H |
NTIS Prices |
PC A03/MF A01 |
Primary Description |
600/02 |
Document Type |
NT |
Cataloging Source |
NTIS/MT |
Control Number |
409118146 |
Origin |
NTIS |
Type |
CAT |