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OLS Field Name OLS Field Data
Main Title Discussion Summary: Application of Biotechnology to the Systematics and Monitoring of Biocontrol Agents.
Author Kawanishi, C. Y. ; Hemming, B. C. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC. ;Monsanto Agricultural Co., St. Louis, MO. Technology Div.
Publisher 1989
Year Published 1989
Report Number EPA/600/D-89/149;
Stock Number PB90-112764
Additional Subjects Deoxyribonucleic acids ; Bacteria ; Enterobacteriaceae ; Biotechnology ; Gene amplification ; Ribosomal RNA
Library Call Number Additional Info Location Last
NTIS  PB90-112764 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 03/10/1990
Collation 7p
Abstract The report is a general discussion of the application of biotechnology to the systematics and monitoring of microbial agents used in biotechnology. Present conventional methods such as microscopy, selective media, marker utilization, etc., were described. Two newer methods, rRNA sequencing and DNA amplification techniques were described and their usefulness in identifying and monitoring microbes used in biotechnology discussed. These were concluded to be the most useful new techniques. Automated methods for bacteria identification and quantification were discussed. These methods were shown to be in need of adaptation to agricultural agents. The relevance of these methodologies is to the Agency's regulation of biotechnology products.
Supplementary Notes Prepared in cooperation with Monsanto Agricultural Co., St. Louis, MO. Technology Div.
NTIS Title Notes Symposium paper.
Category Codes 57K; 57F
NTIS Prices PC A02/MF A01
Primary Description 600/10
Document Type NT
Cataloging Source NTIS/MT
Control Number 935219905
Origin NTIS
Type CAT