Main Title |
Stability of the Mutagenicity in Stored Cigarette Smokers' Urine and Extract. |
Author |
Williams, R. W. ;
Watts, R. ;
Inmon, J. ;
Pasley, T. ;
Claxton, L. ;
|
CORP Author |
Environmental Health Research and Testing, Inc., Research Triangle Park, NC.;Health Effects Research Lab., Research Triangle Park, NC. Genetic Toxicology Div. |
Publisher |
c1990 |
Year Published |
1990 |
Report Number |
EPA-68-02-4456; EPA/600/J-90/458; |
Stock Number |
PB91-183319 |
Additional Subjects |
Mutagens ;
Smoking ;
Urine ;
Liquid chromatography ;
Mutagenicity tests ;
Bioassay ;
Salmonella typhimurium ;
Dose-response relationships ;
Reprints ;
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
NTIS |
PB91-183319 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Collation |
5p |
Abstract |
Urine from cigarette smokers was analyzed for the effect upon mutagenic activity when stored for as long as 175 days. Frozen aliquots of urine were thawed out at various time points in the study and prepared for bioassay. These urine extracts were not bioassayed immediately, but rather refrozen until all of the unprocessed urine samples had eventually been prepared for bioassay. All extracts were obtained using cyanopropyl solid phase extraction techniques. At the end of 175 days, all extracts were bioassayed using a microsuspension assay of Salmonella typhimurium TA98. Urine from smokers was found to be mutagenic (14.4-30.9 revertants/ml equivalent) while a control set of urine from nonsmokers was not. Data from the storage study when analyzed by analysis of variance techniques indicated no statistical loss of mutagens occurred over the 175-day period although near significance was observed (P = 0.054). This near significance was the result of decreasing mutant response as storage time increased for two of the higher doses tested. (Copyright (c) 1990 Wiley-Liss, Inc.) |
Supplementary Notes |
Pub. in Environmental and Molecular Mutagenesis, v16 p246-249 Sep 90. Sponsored by Health Effects Research Lab., Research Triangle Park, NC. Genetic Toxicology Div. |
NTIS Title Notes |
Journal article. |
Title Annotations |
Reprint: Stability of the Mutagenicity in Stored Cigarette Smokers' Urine and Extract. |
Category Codes |
57Y; 57F |
NTIS Prices |
PC A01/MF A01 |
Primary Description |
600/10 |
Document Type |
NT |
Cataloging Source |
NTIS/MT |
Control Number |
117927333 |
Origin |
NTIS |
Type |
CAT |