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Main Title Use of Enzyme-Linked Immunosorbent Assays (ELISA) for the Determination of Triton X Nonionic Detergents.
Author Wie, S. I. ; Hammock., B. D. ;
CORP Author California Univ., Davis.;Health Effects Research Lab., Research Triangle Park, NC.
Year Published 1982
Report Number EPA-R-806447; EPA/600/J-82/465;
Stock Number PB86-219029
Additional Subjects Immunosorbent assays ; Detergents ; Enzyme linked immunosorbent assays ; Nonionic detergents
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NTIS  PB86-219029 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 11p
Abstract An enzyme-linked immunosorbent assay (ELISA) for 4-t-octylphenyl ethoxylates such as Triton X-100 was developed. Both the 4-t-octylphenyl and the ethoxylate moiety were required for antibody recognition since members of the Triton N series showed low cross-reactivity, and polyethylene glycol polymers as well as a variety of other neutral and ionic surfactants and pesticides showed no cross-reactivity. The ELISA was sensitive in the low nanogram-per-milliliter range and was highly reproducible. It was shown to be capable of analyzing the active ingredients in vaginal contraceptives and Triton X-100 in the presence of proteins. Immunoassays thus offer advantages in the analysis of such complex mixtures.
Supplementary Notes Sponsored by Health Effects Research Lab., Research Triangle Park, NC.
Availability Notes Pub. in Analytical Biochemistry, v125 n2 p168-176 Sep 83.
NTIS Title Notes Journal article.
PUB Date Free Form 1982
Category Codes 6A; 57B
NTIS Prices PC A02/MF A01
Primary Description 600/11
Document Type NT
Cataloging Source NTIS/MT
Control Number 625517732
Origin NTIS
Type CAT