Main Title |
Genetic Analysis of the aggA Locus Involved in Agglutination and Adherence of 'Pseudomonas putida', a Beneficial Fluorescent Pseudomonad. |
Author |
Buell, C. R. ;
Anderson, A. J. ;
|
CORP Author |
Utah Agricultural Experiment Station, Logan.;Environmental Research Lab., Gulf Breeze, FL. |
Publisher |
c1992 |
Year Published |
1992 |
Report Number |
PAPER-4152; EPA/600/J-93/219; |
Stock Number |
PB93-205029 |
Additional Subjects |
Pseudomonas putida ;
Bacterial genes ;
Agglutination ;
Open reading frames ;
Bacterial adhesion ;
Deoxyribonucleic acids ;
Molecular sequence data ;
Sequence deletion ;
Genetic complementation test ;
Glycoproteins ;
Phenotype ;
Reprints ;
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
NTIS |
PB93-205029 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Collation |
11p |
Abstract |
An isolate of Pseudomonas putida, which rapidly adheres to plant roots, is agglutinated by a glycoprotein from root surfaces. Agglutination is prevented and adherence to the root surface is diminished by Tn5 insertion in mutant 5123. Two cosmid clones from wild type P. putida and a 2.7-kbp EcoRI-HindIII subclone present in both cosmid clones restored agglutinability to wild type levels in transconjugants of the nonagglutinable (Agg-) Tn5 mutant 5123. These three clones increased agglutinability in transconjugants of the parental Agg+ isolate. The 2.7-kbp EcoRI-HindIII subclone restored adherence to bean root surfaces of 5123 to wild type levels in a short-term binding assay. Deletion analysis of the 2.7-kbp fragment indicated only 1.45 kbp was necessary for complementation of agglutinability in 5123. The sequence, termed the aggA locus, contains an open reading frame of 1,356 nucleotides encoding a predicted 50,509-Da protein. The distribution of the aggA locus in plant-associated bacteria, as detected through Southern hybridization, is limited to bacteria that express the agglutination phenotype. |
Supplementary Notes |
Pub. in Molecular Plant-Microbe Interactions, v5 n2 p154-162 1992. Sponsored by Environmental Research Lab., Gulf Breeze, FL. |
NTIS Title Notes |
Journal article. |
Title Annotations |
Reprint: Genetic Analysis of the aggA Locus Involved in Agglutination and Adherence of 'Pseudomonas putida', a Beneficial Fluorescent Pseudomonad. |
Category Codes |
57K; 57F |
NTIS Prices |
PC A03/MF A01 |
Primary Description |
600/04 |
Document Type |
NT |
Cataloging Source |
NTIS/MT |
Control Number |
323828391 |
Origin |
NTIS |
Type |
CAT |