Main Title |
Phenoxyacetic Acid Degradation by the 2,4-Dichlorophenoxyacetic Acid (TFD) Pathway of Plasmid pJP4: Mapping and Characterization of the TFD Regulatory Gene, tfdR. |
Author |
Harker, A. L. ;
Olsen, R. H. ;
Seidler, R. J. ;
|
CORP Author |
Corvallis Environmental Research Lab., OR. ;Oregon State Univ., Corvallis. ;Michigan Univ., Ann Arbor. Medical School. |
Publisher |
c1989 |
Year Published |
1989 |
Report Number |
EPA/600/J-89/129; |
Stock Number |
PB90-111170 |
Additional Subjects |
Biodeterioration ;
Deoxyribonucleic acids ;
Alcaligenes ;
Pseudomonas ;
Mutation ;
Reprints ;
2-4 Dichlorophenoxy acetic acid ;
Plasmids ;
Regulator genes ;
Restriction mapping ;
Phenoxyacetic acid ;
Agargel electrophoresis ;
Alcaligenes eutrophics
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
NTIS |
PB90-111170 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Collation |
9p |
Abstract |
Plasmid pJP4 enables Alcaligenes eutrophus JMP134 to degrade 3-chlorobenzoate and 2,4-dichlorophenoxyacetic acid (TFD). Plasmid pR0101 is a derivative of pJP4 obtained by insertion of Tn1721 into a nonessential region of pJP4. Plasmid pR0101 was transferred by conjugation to several Pseudomonas strains and to A. eutrophus AEO106, a cured isolate of JMP134. AEO106(pR0101) and some Pseudomonas transconjugants grew on TFD. Transconjugants with a chromosomally encoded phenol hydroxylase also degraded phenoxyacetic acid (PAA) in the presence of an inducer of the TFD pathway, namely, TFD or 3-chlorobenzoate. A mutant of one such phenol-degrading strain, Pseudomonas putida PP0300(pR0101), grew on PAA as the sole carbon source in the absence of inducer. This isolate carried a mutant plasmid, designated pR-103, derived from pR0101 through the deletion of a 3.9-kilobase DNA fragment. Plasmid pR0103 constitutively expressed the TFD pathway, and this allowed the metabolism of PAA in the absence of the inducer, TFD. Complementation of pR0103 in trans by a DNA fragment corresponding to the fragment deleted in pR0101 indicates that a negative control-regulatory gene (tfdR) is located on the BamHI E fragment of pR0101. (Copyright (c) 1989, American Society for Microbiology.) |
Supplementary Notes |
Pub. in Jnl. of Bacteriology, v171 n1 p314-320 1989. Prepared in cooperation with Oregon State Univ., Corvallis, and Michigan Univ., Ann Arbor. Medical School. |
NTIS Title Notes |
Journal article. |
Title Annotations |
Reprint: Phenoxyacetic Acid Degradation by the 2,4-Dichlorophenoxyacetic Acid (TFD) Pathway of Plasmid pJP4: Mapping and Characterization of the TFD Regulatory Gene, tfdR. |
Category Codes |
57F; 99D |
NTIS Prices |
PC A02/MF A01 |
Primary Description |
600/02 |
Document Type |
NT |
Cataloging Source |
NTIS/MT |
Control Number |
935218407 |
Origin |
NTIS |
Type |
CAT |