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RECORD NUMBER: 136 OF 397

OLS Field Name OLS Field Data
Main Title Evaluation of a Method to Measure Conjugal Transfer of Recombinant DNA in Soil Slurries.
Author Walter, M. V. ; Porteous, L. A. ; Seidler., R. J. ;
CORP Author Corvallis Environmental Research Lab., OR. ;NSI Technology Services Corp., Corvallis, OR. ;Oregon State Univ., Corvallis. Dept. of Microbiology.
Publisher c1989
Year Published 1989
Report Number EPA/600/J-89/480;
Stock Number PB91-117580
Additional Subjects Microorganisms ; Pseudomonas ; Soil microbiology ; pH ; Nutrients ; Reprints ; Recombinant DNA ; Genetic engineering ; Genetic conjugation ; Plasmids ; Risk assessment ; Transfection
Holdings
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NTIS  PB91-117580 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 03/04/1991
Collation 8p
Abstract The report evaluates a method to detect conjugal DNA plasmid transfer in soil slurries under various environmental conditions. Donor Pseudomonas cepacia containing pR388::Tn1721 and P. cepacia recipient cultures were co-incubated in soil slurries containing autoclaved or natural soil and treated with one or more of 14 experimental conditions. Conjugal mating frequency (transconjugants per initial donor) ranged from 4.8 X ten to the minus 1 to 1.9 X ten to the minus seventh. Highest numbers of transconjugants, 1.5 X ten to the seventh colony forming units/ml soil slurry, were observed following incubation at 35C with an enriched nutrient supplement added to the soil. Low numbers of transconjugants, ten to the third colony forming units/ml soil slurry, were observed when mating pairs were subjected to low nutrient or pH stress even though initial donor and recipient populations were maintained at high levels. The test system provides a simple way to estimate effects of changing environmental factors on plasmid transfer rates and on the survival of recombinant microorganisms. By use of soil collected from sites proposed to receive genetically engineered microorganisms, preliminary risk assessments can be obtained regarding the potential for gene transfer and microorganism survival with this soil slurry test system. (Copyright (c) Springer-Verlag New York Inc. 1989)
Supplementary Notes Pub. in Current Microbiology, v19 p365-370 1989. Prepared in cooperation with NSI Technology Services Corp., Corvallis, OR., and Oregon State Univ., Corvallis. Dept. of Microbiology.
NTIS Title Notes Journal article.
Title Annotations Reprint: Evaluation of a Method to Measure Conjugal Transfer of Recombinant DNA in Soil Slurries.
Category Codes 57K; 57F
NTIS Prices PC A02/MF A01
Primary Description 600/02
Document Type NT
Cataloging Source NTIS/MT
Control Number 101124258
Origin NTIS
Type CAT