Main Title |
Microcosm Method to Assess Survival of Recombinant Bacteria Associated with Plants and Herbivorous Insects (Journal Version). |
Author |
Armstrong, J. L. ;
Knudsen, G. R. ;
Seidler, R. J. ;
|
CORP Author |
Corvallis Environmental Research Lab., OR. ;Oregon State Univ., Corvallis. Dept. of Microbiology. |
Publisher |
c1987 |
Year Published |
1987 |
Report Number |
EPA/600/J-87/455; |
Stock Number |
PB89-134613 |
Additional Subjects |
Genetic intervention ;
Recombinant DNA ;
Plants(Botany) ;
Ecosystems ;
Population dynamics ;
Survival ;
Plasmids ;
Reprints ;
Pseudomonas cepacia ;
Cutworms ;
Peridroma saucia
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
NTIS |
PB89-134613 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Collation |
6p |
Abstract |
A microcosm method was developed to investigate survival and fate of genetically engineered bacteria associated with plant surfaces and a plant-feeding insect, the variegated cutworm, Peridroma saucia. Larvae on radish plants in microcosms were sprayed with nonrecombinant Pseudomonas cepacia and a recombinant strain of P. cepacia carrying the transmissible plasmid R388::Tn1721. Immediately after spraying, P. cepacia comprised about 20%-30% of the total population on leaves. After 2 days, the total epiphytic population had increased approximately fourfold, while the P. cepacia strains had decreased to 2%-30% of their initial numbers. Plasmid transfer from P. cepacia R388::Tn 1721 to the nonrecombinant recipient P. cepacia strain was not observed. (Copyright (c) Springer-Verlag New York Inc. 1987.) |
Supplementary Notes |
Pub. in Current Microbiology, v15 p229-232 1987. Prepared in cooperation with Oregon State Univ., Corvallis. Dept. of Microbiology. |
NTIS Title Notes |
Journal article. |
Title Annotations |
Reprint: Microcosm Method to Assess Survival of Recombinant Bacteria Associated with Plants and Herbivorous Insects (Journal Version). |
Category Codes |
57F#; 57K; 57C |
NTIS Prices |
PC A02/MF A01 |
Primary Description |
600/02 |
Document Type |
NT |
Cataloging Source |
NTIS/MT |
Control Number |
905520984 |
Origin |
NTIS |
Type |
CAT |