||Immunofluorescence Detection of 'Cryptosporidium' Oocysts in Fecal Smears.
Stibbs, H. H. ;
Ongerth, J. E. ;
||Washington Univ., Seattle. School of Public Health and Community Medicine.;Health Effects Research Lab., Research Triangle Park, NC.
Para sitology ;
Methyl sulfoxide ;
Biological stains ;
Macaca nemestrina ;
Rosaniline dyes ;
||Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy.
||An indirect fluorescent antibody (IFA) procedure was developed for the detection of Cryptosporidium sp. oocysts in human, nonhuman primate, and bovine fecal smears. The procedure, which takes about 90 min to perform, involves the use of a rabbit antiserum against Cryptosporidium oocysts isolated from dairy cattle. Cross-specificity testing of the IFA method revealed no reactivity with yeasts, various amoebae, Giardia lamblia, Chilomastix sp., or Blastocystis sp. and only very weak cross-reactivity with coccidian oocysts of other genera. IFA detection of oocysts in human and nonhuman primate fecal smears was far more sensitive than was dimethyl sulfoxide-carbolfuchsin staining. Moreover, IFA detection was comparable in sensitivity to auramine O staining with samples of high oocyst concentration and somewhat more sensitive than auramine O with sample containing relatively few oocysts. The IFA procedure may be useful in the clinical diagnosis of human and animal cryptosporidiosis and also in the detection of oocysts in environmental samples. (Copyright (c) 1986, American Society for Microbiology.)
||Pub. in Jnl. of Clinical Microbiology, v24 n4 p517-521 Oct 86. Sponsored by Health Effects Research Lab., Research Triangle Park, NC.
|NTIS Title Notes
||Reprint: Immunofluorescence Detection of 'Cryptosporidium' Oocysts in Fecal Smears.
|PUB Date Free Form
||PC A02/MF A01