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OLS Field Name OLS Field Data
Main Title Animal source identification using a cryptosporidium DNA characterization technique [electronic resource] /
Author Royer, Michael D. ; Royer, M. ; Xiao, L. ; Lal, A.
Other Authors
Author Title of a Work
Xiao, Lihua.
Lal, Altaf.
CORP Author National Risk Management Research Lab., Cincinnati, OH. ;Centers for Disease Control and Prevention, Atlanta, GA.
Publisher U.S. Environmental Protection Agency, Office of Research and Development, National Risk Management Research Laboratory ; U.S. Department of Health and Human Services, Centers for Disease Control and Prevention, Division of Parasitic Diseases, National Center for Infectious Diseases,
Year Published 2002
Report Number EPA/600/R-03/047
Stock Number PB2003-107722
Subjects Water--Sampling. ; Cryptosporidium. ; DNA.
Additional Subjects Water sampling ; Ribosomal DNA ; Oocysts ; Characterization ; Differentiation ; Detection ; Identification ; Genotype ; Hosts ; Species ; Specificity ; Disease prevention ; Cryptosporidiosis ; Molecular methods ; Source animal types ; Host ranges
Internet Access
Description Access URL
Abstract http://www.epa.gov/nrmrl/pubs/600r03047/600r03047.htm
Library Call Number Additional Info Location Last
NTIS  PB2003-107722 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 10/24/2003
Collation 1 online resource (vii, 19 p). : ill., charts, digital, PDF file.
Abstract This document summarizes the application of a particular molecular method to improve detection and differentiation of species and genotypes of Cryptosporidium oocysts found in environmental samples. Of particular interest is the methods potential for determining the source animal types of oocysts in water samples. The molecular method is a nested polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) procedure that characterizes the small sub-unit (SSU) ribosomal RNA gene. The method was previously developed for characterizing oocyst DNA from clinical samples. The current pro ject explores the methods applicability to environmental water samples, which have greater diversity of oocyst species and strains, lower concentrations of oocysts, and different interferents than clinical samples. Results include demonstrating that the method is capable of detection and differentiation of at least 10 species and 22 genotypes of Cryptosporidium; method sensitivity demonstrated to a single oocyst with laboratory samples; and detection and differentiation of oocysts from oyster gill washings and hemolymph, storm water, surface water, and raw waste water. The methods capability to determine an oocysts source animal type was demonstrated by identification in environmental water samples of host-adapted Cryptosporidium species and genotypes that were consistent with the source animal types (i.e., humans, farm animals, wildlife, and/or pets) inhabiting the sampled watersheds.
Notes Title from title screen (viewed on Feb. 18, 2011). "EPA/600/R-03/047." Includes bibliographical references (p. 17-18). "September 2002."
Place Published Cincinnati, Ohio Atlanta, Ga.
Supplementary Notes Prepared in cooperation with Centers for Disease Control and Prevention, Atlanta, GA.
Availability Notes Also available on CD-ROM. Product reproduced from digital image. Order this product from NTIS by: phone at 1-800-553-NTIS (U.S. customers); (703)605-6000 (other countries); fax at (703)605-6900; and email at orders@ntis.gov. NTIS is located at 5285 Port Royal Road, Springfield, VA, 22161, USA.
Access Notes Mode of access: World Wide Web.; System requirements: Adobe Acrobat Reader.
Corporate Au Added Ent National Center for Infectious Diseases (U.S.). Division of Parasitic Diseases.; National Risk Management Research Laboratory (U.S.)
PUB Date Free Form 2002
Category Codes 57F
NTIS Prices PC A03/MF A01
Primary Description 600/14
BIB Level m
Medium electronic resource
Cataloging Source OCLC/T
Control Number 029600960
OCLC Time Stamp 20110218164740
Language eng
Origin NTIS
OCLC Rec Leader 01767nam 2200397Ia 45020