Main Title |
Quantitative Changes in the Synaptic Vesicle Proteins Synapsin I and p38 and the Astrocyte-Specific Protein Glial Fibrillary Acidic Protein are Associated with Chemical-Induced Injury to the Rat Central Nervous System (Journal Version). |
Author |
Brock, T. O. ;
O'Callaghan, J. P. ;
|
CORP Author |
Health Effects Research Lab., Research Triangle Park, NC. |
Publisher |
1987 |
Year Published |
1987 |
Report Number |
EPA/600/J-87/394; |
Stock Number |
PB89-106041 |
Additional Subjects |
Brain injuries ;
Toxicity ;
Neurochemistry ;
Nerve tissue proteins ;
Glial fibrillary acidic protein ;
Astrocytes ;
Synaptic vesicles ;
Pathology ;
Tin organic compounds ;
Hippocampus ;
Frontal cortex ;
Reprints ;
Synapsin I ;
P38 protein
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
NTIS |
PB89-106041 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Collation |
15p |
Abstract |
Measurements of neuron-specific and glia-specific proteins were used to characterize chemical-induced injury to the rat CNS. Trimethyltin (TMT), a neurotoxicant which preferentially damages neurons in limbic structures, was employed to produce consistent, time-dependent, dose-related, cell-type-specific alterations in CNS morphology. Brain weights and histology were used to verify the cytopathological effects of TMT. Accompanying changes in two synaptic vesicle-associated proteins, synapsin I and p38, and the astrocyte-associated protein, glial fibrillary acidic protein (GFAP), were measured by radioimmunoassay (RIA). Immunohistochemistry of GFAP and incorporation of 3H-thymidine into GFAP-positive astrocytes also were used to characterize astrocytic responses to TMT-induced injury. Finally, quantitative two-dimensional PAGE was employed to detect additional proteins affected by TMT. Acute administration of TMT caused large dose- and time-dependent decreases in synapsin I and p38 in hippocampus; the same proteins were largely unaffected in a non-limbic structure, the frontal cortex. |
Supplementary Notes |
Pub. in the Jnl. of Neuroscience, v7 p931-942 Apr 87. |
NTIS Title Notes |
Journal article. |
Title Annotations |
Reprint: Quantitative Changes in the Synaptic Vesicle Proteins Synapsin I and p38 and the Astrocyte-Specific Protein Glial Fibrillary Acidic Protein are Associated with Chemical-Induced Injury to the Rat Central Nervous System (Journal Version). |
Category Codes |
57Y; 57B; 57S |
NTIS Prices |
PC A03/MF A01 |
Primary Description |
600/11 |
Document Type |
NT |
Cataloging Source |
NTIS/MT |
Control Number |
834422057 |
Origin |
NTIS |
Type |
CAT |