||X-ray Microanalysis of Calcium, Potassium, and Phosphorus in Liver Mitochondria Stressed by Carbon Tetrachloride.
Millette, J. R. ;
Allenspach, A. L. ;
Clark, P. J. ;
McCauley, P. T. ;
Washington, I. S. ;
||Health Effects Research Lab., Research Triangle Park, NC. ;Miami Univ., Oxford, OH. Dept. of Zoology.
Carbon tetrachloride ;
X ray analysis ;
Environmental surveys ;
In vivo analysis ;
Laboratory animals ;
In vitro analysis ;
Chemical analysis ;
Environmental health ;
||Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy.
||Previous measurements of elemental concentrations in liver mitochondria have generally required homogenation and fractionation of liver tissue, a procedure in which it is difficult to rule out ion movement between subcellular units. New techniques involving cryo-ultramicrotomy of rapidly frozen tissue, high resolution scanning transmission electron microscopy and X-ray microanalysis were used to measure those elements in rat liver mitochondria reported to have changed following oral administration of carbon tetrachloride (CCl4). Increases in liver mitochondrial calcium were found 24 hours following intoxication by CCl4. Significant early (2 hours) mitochondrial increases in potassium and phosphorus were found following administration of CCl4. The electron microscope technique using quick frozen samples promises to allow measurement of intracellular ionic concentrations under virtually lifelike conditions.
||Prepared in cooperation with Miami Univ., Oxford, OH. Dept. of Zoology.
||Pub. in Jnl. of Analytical Toxicology, v9 p145-151 Jul/Aug 85.
|NTIS Title Notes
||Reprint: X-ray Microanalysis of Calcium, Potassium, and Phosphorus in Liver Mitochondria Stressed by Carbon Tetrachloride.
|PUB Date Free Form
||6T; 6E; 7D; 57Y; 99A; 68G; 57U
||Not available NTIS