| Main Title |
'Giardia' Detection in Water Systems. |
| Author |
Sauch, J. F. ;
|
| CORP Author |
Health Effects Research Lab., Cincinnati, OH. |
| Year Published |
1986 |
| Report Number |
EPA/600/D-86/234; |
| Stock Number |
PB87-104493 |
| Additional Subjects |
Giardia ;
Water supply ;
Gastro intestinal diseases ;
Detection ;
Immune serums ;
Cysts ;
Identifying ;
Biological stains ;
Fluorescence ;
Cell morphology ;
Chlorination ;
Tests ;
Pennsylvania ;
Massachusetts ;
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB87-104493 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
11p |
| Abstract |
A method has been developed combining immunofluorescence and phase-contrast to microscopically locate and identify Giardia cysts among particulates filtered from water samples. Selective staining of cysts with anti-Giardia antiserum and a fluorochrom enhances the contrast between cysts and other sample constituents and allows quick location of cysts by their green fluorecence. Their identity can then be confirmed with phase-contrast observation of size, shape, and internal morphology. The method was successfully tested during recent outbreaks in Pennsylvania and Massachusetts. However, when chlorinated water samples were tested, confirmation of presumptive Giardia cysts was difficult or impossible. |
| PUB Date Free Form |
Oct 86 |
| Category Codes |
57K; 57U; 48B |
| NTIS Prices |
PC A02/MF A01 |
| Primary Description |
600/11 |
| Document Type |
NT |
| Cataloging Source |
NTIS/MT |
| Control Number |
635314807 |
| Origin |
NTIS |
| Type |
CAT |
