||Aluminum Alters Calcium Transport in Plasma Membrane and Endoplasmic Reticulum from Rat Brain.
Mundy, W. R. ;
Kodavanti, P. R. S. ;
Dulchinos, V. F. ;
Tilson., H. A. ;
||Health Effects Research Lab., Research Triangle Park, NC. Neurotoxicology Div.
Biological transport ;
Endoplasmic reticulum ;
Ca(2+) Mg(2+)-ATPase ;
Signal transduction ;
Cell membrane ;
||Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy.
||Calcium is actively transported into intracellular organelles and out of the cytoplasm by Ca(2+)/Mg(2+)-ATPases located in the endoplasmic reticulum and plasma membranes. The authors studied the effects of aluminum on calcium transport in the adult rat brain. They examined (45)Ca-uptake in microsomes and Ca(2+)-ATPase activity in microsomes and synaptosomes isolated from the frontal cortex and cerebellum of adult male Long-Evans rats. ATP-dependent (45)Ca-uptake was similar in microsomes from both brain regions. The addition of 50-800 microM AlCl3 resulted in a concentration-dependent inhibition of (45)Ca-uptake. Mg(2+)-dependent Ca(2+)-ATPase activity was significantly lower in synaptosomes compared to microsomes in both frontal cortex and cerebellum. In contrast to the uptake studies, AlCl3 stimulated Mg(2+)-dependent Ca(2+)-ATPase activity in both microsomes and synaptosomes from both brain regions. To determine the relationship between aluminum and Mg(2+), the authors measured ATPase activity in the presence of increasing concentrations of Mg(2+) or AlCl3. Maximal ATPase activity was obtained between 3 and 6 mM Mg(2+). (Copyright (c) 1994 VCH Publishers, Inc.)
||Pub. in Jnl. of Biochemical Toxicology, v9 n1 p17-23 Feb 94.
|NTIS Title Notes
||Reprint: Aluminum Alters Calcium Transport in Plasma Membrane and Endoplasmic Reticulum from Rat Brain.
||57Y; 57B; 57F
||PC A02/MF A01