||Biodegradation of DDT (1,1,1-Trichloro-2,2-Bis(4-Chlorophenyl)Ethane) by the White Rot Fungus 'Phanerochaete chrysosporium'.
Bumpus, J. A. ;
Aust, S. D. ;
||Michigan State Univ., East Lansing. Dept. of Biochemistry.;Environmental Protection Agency, Cincinnati, OH. Risk Reduction Engineering Lab.
Culture media ;
Mass fragmentography ;
High pressure liquid chromatography ;
Carbon 14 ;
Phanerochaete chrysosporium ;
Pleurotus ostreatus ;
Phellinus weirii ;
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||Extensive biodegradation of 1,1,1-trichloro-2,2bis(4-chlorophenyl)ethane (DDT) by the white rot fungus Phanerochaete chrysosporium was demonstrated by disappearance and mineralization of (14C) DDT in nutrient nitrogen-deficient cultures. Mass balance studies demonstrated the formation of polar and water-soluble metabolites during degradation. Hexane-extractable metabolites identified by gas chromatography-mass spectrometry included 1,1-dichloro-2,2-bis(4-chlorophenyl)ethane(DDD), 2,2,2-trichloro-1,1-bis(4-chlorophenyl)ethanol (dicofol), 2,2-dichloro-1,1-bis(4-chlorophenyl) ethanol (FW-152), and 4,4'-dichlorobenzophenone (DBP). DDD was the first metabolite observed; it appeared after 3 days of incubation and disappeared from culture upon continued incubation. This, as well as the fact that ((14)C) dicofol was mineralized, demonstrates that intermediates formed during DDT degradation are also metabolized. These results demonstrate that the pathway for DDT degradation in P. chrysosporium is clearly different from the major pathway proposed for microbial or environmental degradation of DDT. Like P. chrysosporium ME-446 and BKM-F-1767, the white rot fungi Pleurotus ostreatus, Phellinus weirii, and Polyporus versicolor also mineralized DDT. (Copyright (c) 1987 American Society for Microbiology.)
||Pub. in Applied and Environmental Microbiology, v53 n9 p2001-2008 Sep 87. See also PB93-191575 and PB93-191567. Sponsored by Environmental Protection Agency, Cincinnati, OH. Risk Reduction Engineering Lab.
|NTIS Title Notes
||Reprint: Biodegradation of DDT (1,1,1-Trichloro-2,2-Bis(4-Chlorophenyl)Ethane) by the White Rot Fungus 'Phanerochaete chrysosporium'.
||57K; 57B; 68E
||PC A02/MF A01