Main Title |
Immunocytochemical Localization of Calcium/Calmodulin-Dependent Protein Kinase II in Rat Brain. |
Author |
Ouimet, C. C. ;
McGuinness, T. L. ;
Greengard, P. ;
|
CORP Author |
Rockefeller Univ., New York.;Health Effects Research Lab., Research Triangle Park, NC. |
Year Published |
1984 |
Report Number |
EPA-R-810608; EPA/600/J-84/385; |
Stock Number |
PB87-145520 |
Additional Subjects |
Calcium ;
Rats ;
Laboratory animals ;
Reprints ;
Protein kinase ;
Calmodulin ;
Immunocytochemistry
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
NTIS |
PB87-145520 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Collation |
8p |
Abstract |
Calcium/calmodulin-dependent protein kinase II (CaM kinase II) is a prominent enzyme in mammalian brain capable of phosphorylating a variety of substrate proteins. In the present investigation, the subcellular and regional distribution of CaM kinase II has been studied by light and electron microscopic immunocytochemistry using an antibody that recognizes the Mr 50,000 and 60,000/58,000 subunits of the enzyme. Light microscopy demonstrates strong immunoreactivity in axons. Electron microscopy, in addition to confirming light microscopic observations, reveals moderate immunoreactivity in spines and weak immunoreactivity in nerve terminals. An accumulation of immunoreaction product is also present on postsynaptic densities. The presence of CaM kinase II in diverse structures throughout the neuron supports the view that this enzyme may be involved in mediating a variety of caldium-dependent physiological processes. CaM kinase II immunoreactivity is present in neurons throughout the brain, but a marked regional variation in the strength of the immunoreactivity exists. |
Supplementary Notes |
Pub. in Proceedings of the National Academy of Science USA, v81, p5604-5608, Sep 84. Sponsored by Health Effects Research Lab., Research Triangle Park, NC. |
NTIS Title Notes |
Journal article. |
Title Annotations |
Reprint: Immunocytochemical Localization of Calcium/Calmodulin-Dependent Protein Kinase II in Rat Brain. |
PUB Date Free Form |
c1984 |
Category Codes |
57B |
NTIS Prices |
PC A02/MF A01 |
Primary Description |
600/11 |
Document Type |
NT |
Cataloging Source |
NTIS/MT |
Control Number |
711419604 |
Origin |
NTIS |
Type |
CAT |