| Main Title |
Specific Proteins and Their Application in Wastewater Treatment. |
| Author |
Furlong, C. E. ;
Sundstrom, J. A. ;
Weiler, E. B. ;
Cheung, P. W. ;
Yin, J. ;
|
| CORP Author |
Washington Univ., Seattle. ;California Univ., Berkeley. Dept. of Chemical Engineering.;Environmental Protection Agency, Cincinnati, OH. Risk Reduction Engineering Lab. |
| Publisher |
Oct 88 |
| Year Published |
1988 |
| Report Number |
EPA-R-811948; EPA/600/D-88/228; |
| Stock Number |
PB89-119721 |
| Additional Subjects |
Water pollution abatement ;
Escherichia coli ;
Proteins ;
Cadmium ;
Phosphates ;
Polymers ;
Peptides ;
Waste water treatment ;
Bioreactors ;
Carrier proteins ;
Metallothionein
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB89-119721 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
33p |
| Abstract |
The purpose of the research was to test the feasibility of using immobilized proteins as highly specific adsorbers of pollutants in waste streams. The Escherichia coli periplasmic phosphate-binding protein served as both a model system for determining the feasibility of such an approach and at the same time was used to produce a cycling ligand adsorber capable of efficiently scrubbing phosphate from a feed stream. Three different procedures for the removal of cadmium from feed streams were developed in this project. A bioreactor for continuous protein production was developed to minimize the cost of protein production. The bioreactor contained immobilized, non-growing E. coli cells. The cell line had been mutagenized and selected for the direct secretion of periplasmic proteins into the medium. The cells contained the gene that encodes the phosphate-binding protein. |
| Supplementary Notes |
Prepared in cooperation with California Univ., Berkeley. Dept. of Chemical Engineering. Sponsored by Environmental Protection Agency, Cincinnati, OH. Risk Reduction Engineering Lab. |
| NTIS Title Notes |
Rept. for Oct 84-Dec 87. |
| Category Codes |
68D; 57B; 57K |
| NTIS Prices |
PC A03/MF A01 |
| Primary Description |
600/14 |
| Document Type |
NT |
| Cataloging Source |
NTIS/MT |
| Control Number |
901217259 |
| Origin |
NTIS |
| Type |
CAT |
