Main Title |
Method of Euthanasia Does Not Affect Sperm Motility in the Laboratory Rat. (Technical Note). |
Author |
Slott, V. L. ;
Linder, R. E. ;
Dyer, C. J. ;
|
CORP Author |
ManTech Environmental Technology, Inc., Research Triangle Park, NC.;Health Effects Research Lab., Research Triangle Park, NC. Reproductive Toxicology Branch. |
Publisher |
cAug 94 |
Year Published |
1994 |
Report Number |
EPA-68D20056; EPA/600/J-94/541; |
Stock Number |
PB95-148037 |
Additional Subjects |
Sperm motility ;
Euthanasia ;
Anesthesia ;
Laboratory animals ;
Rats ;
Carbon dioxide ;
Ethyl ether ;
Halothane ;
Reprints ;
Nembutal
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
NTIS |
PB95-148037 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Collation |
6p |
Abstract |
To determine if anesthetic agents used in laboratory animal euthanasia affected sperm motion parameters, rats (n = 10 per group) were euthanized by one of 5 different methods: decapitation alone, or decapitation following either ether, halothane, or Nembutal anesthesia, or CO2 asphyxiation. Sperm were collected from the distal cauda epididymis, diluted, and videotaped for computer-aided sperm analysis (CASA; HTM-2030, Hamilton-Thorn Research, Beverly, MA). The percentage of motile sperm (MOT), their straight-line velocity (VSL), average path velocity (VAP), curvilinear velocity (VCL), linear index (LINX), and linearity (LIN) were measured on greater than or equal to 200 motile sperm per sample. No significant differences in any of these 6 motion parameters were found among the treatment groups. Thus, none of these 5 methos of euthanasia affect sperm motion as assessed by CASA methods, making them equally suitable for use in reproductive toxicology studies. (Copyright (c) 1994 Elsevier Science Ltd.) |
Supplementary Notes |
Pub. in Reproductive Toxicology, v8 n4 p371-374 Aug 94. Sponsored by Health Effects Research Lab., Research Triangle Park, NC. Reproductive Toxicology Branch. |
NTIS Title Notes |
Journal article. |
Title Annotations |
Reprint: Method of Euthanasia Does Not Affect Sperm Motility in the Laboratory Rat. (Technical Note). |
Category Codes |
57Y; 57S |
NTIS Prices |
PC A02/MF A01 |
Primary Description |
600/10 |
Document Type |
NT |
Cataloging Source |
NTIS/MT |
Control Number |
503414655 |
Origin |
NTIS |
Type |
CAT |