| Main Title |
Chitinase Determinants of 'Vibrio vulnificus': Gene Cloning and Applications of a Chitinase Probe. |
| Author |
Wortman, A. T. ;
Somerville, C. C. ;
Colwell, R. R. ;
|
| CORP Author |
Maryland Univ., College Park. Dept. of Microbiology.;Environmental Research Lab., Gulf Breeze, FL. |
| Year Published |
1986 |
| Report Number |
EPA/600/J-86/196; |
| Stock Number |
PB87-152625 |
| Additional Subjects |
Genetics ;
Substrates ;
Genes ;
Chromosomes ;
Reprints ;
Chitinase ;
Activity(Biology) ;
Vibrio vulnificus
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB87-152625 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
6p |
| Abstract |
To initiate study of the genetic control of chitinolytic activity in vibrios, the chitobiase gene was isolated by cloning chromosomal DNA prepared from Vibrio vulnificus. Chimeric plasmids were constructed from Sau3A I partial digests of chromosomal DNA by ligating 5 to 15-Kilobase fragments into the BamHI site, i.e., in the Tc(sub r) gene, of pBR322 Am(sup r)Tc(sub r). The resulting plasmids were transformed into Escherichia coli DH1. Chitobiase activity of the insert-bearing clones was detected by using a chromogenic substrate, p-nitrophenyl-N-acetyl beta, D-glucosaminide, and confirmed by the appearance of a fluorescent end product from the hydrolysis of 4-methylumbelliferyl-beta, D-N-N'-diacetylchitiobiose. Physical mapping of plasmids containing the chitinase determinants indicate that transcription of these genes in E. coli may be initiated at a V. vulnificus promoter. |
| Supplementary Notes |
Pub. in Applied Environmental Microbiology, v52 n1 p142-145. Sponsored by Environmental Research Lab., Gulf Breeze, FL. |
| NTIS Title Notes |
Journal article. |
| Title Annotations |
Reprint: Chitinase Determinants of 'Vibrio vulnificus': Gene Cloning and Applications of a Chitinase Probe. |
| PUB Date Free Form |
c1986 |
| Category Codes |
57F; 57K |
| NTIS Prices |
PC A02/MF A01 |
| Primary Description |
600/04 |
| Document Type |
NT |
| Cataloging Source |
NTIS/MT |
| Control Number |
712830621 |
| Origin |
NTIS |
| Type |
CAT |
