Main Title |
Gene engineering of yeasts for the degradation of hazardous waste / |
Author |
Loper, John C.
|
CORP Author |
Cincinnati Univ., OH. Coll. of Medicine.;Environmental Protection Agency, Cincinnati, OH. Hazardous Waste Engineering Research Lab. |
Publisher |
U.S. Environmental Protection Agency, Hazardous Waste Engineering Research Laboratory, |
Year Published |
1988 |
Report Number |
EPA/600-M-88-001; Air Force no. ESL-TR-86-52; EPA-R-810605 |
Stock Number |
PB88-182597 |
OCLC Number |
18396082 |
Subjects |
Hazardous wastes--Biodegradation ;
Yeast
|
Additional Subjects |
Hazardous wastes ;
Biodeterioration ;
Yeasts ;
Cytochromes ;
Antibodies ;
Laboratory animals ;
Deoxyribonucleic acids ;
Genetic intervention
|
Internet Access |
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
EHAM |
EPA/600/M-88/001 |
|
Region 1 Library/Boston,MA |
05/25/2016 |
EJBD |
EPA 600-M-88-001 |
|
Headquarters Library/Washington,DC |
07/19/2017 |
ELBD ARCHIVE |
EPA 600-M-88-001 |
In Binder Received from HQ |
AWBERC Library/Cincinnati,OH |
10/04/2023 |
ELBD RPS |
EPA 600-M-88-001 |
repository copy |
AWBERC Library/Cincinnati,OH |
10/17/2014 |
NTIS |
PB88-182597 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Collation |
3 pages ; 28 cm |
Abstract |
The research examined the structure and function of cytochrome P-450 genes in yeast as a model for gene engineering such as eukaryotic P-450 enzymes for biodegradation of hazardous waste by yeasts. Saccharomyces cerevisiae and Candida tropicalis are two yeasts known to produce major P-450 enzymes. These enzymes were purified and antibodies produced in rabbits were then used in the isolation or characterization of clones containing a P-450 gene from each organism. DNA sequence was determined for the gene isolated from S. cerevisiae and for several hundred bases of chromosomal DNA on each side of the gene, and deletion experiments in the promoter region were conducted. The deduced protein sequence from this gene was compared to those of the other known subfamilies of eukaryotic P-450 genes; the several features identified included a new homology region for these protein sequences. The C. tropicalis gene and its promoter region have been partially sequenced. |
Notes |
Caption title. At head of title: Environmental research brief. Shipping list no.: 88-499-P. "Jan. 1988." "EPA/600-M-88-001." "Air Force No. ESL-TR-86-52." |
Place Published |
Cincinnati, OH |
Supplementary Notes |
Sponsored by Environmental Protection Agency, Cincinnati, OH. Hazardous Waste Engineering Research Lab. |
Access Notes |
Also issued online. |
Corporate Au Added Ent |
Hazardous Waste Engineering Research Laboratory. |
PUB Date Free Form |
1988 |
NTIS Prices |
PC A02/MF A01 |
BIB Level |
m |
Medium |
unmediated |
Content |
text |
Carrier |
volume |
Cataloging Source |
OCLC/T |
Merged OCLC records |
1225676880 |
OCLC Time Stamp |
20160419144233 |
Language |
eng |
SUDOCS Number |
EP 1.96:600/M-88/001 |
Origin |
OCLC |
Type |
MERGE |
OCLC Rec Leader |
01384cam 2200397Ia 45020 |