Main Title |
Initial Submission: Detection of Effects of Tributyltin Oxide on Natural Killer-Actiuvity in the Rat Lung, Using a Newly Method with Cover Letter dated 09/29/1992. |
CORP Author |
National Inst. of Public Health, Oslo (Norway).; Elf Atochem North America, Philadelphia, PA.; Environmental Protection Agency, Washington, DC. Office of Toxic Substances. |
Year Published |
1992 |
Report Number |
8EHQ-1092-11326 |
Stock Number |
OTS0571264 |
Additional Subjects |
Toxicology ;
Health effects ;
Tributyltin oxide ;
Biochemistry ;
Mammals ;
Rats ;
Inhalation ;
Oral ;
Diet ;
CAS No 56-35-9 ;
CAS No 118-74-1 ;
CAS No 10028-15-6
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
NTIS |
OTS0571264 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Collation |
41p |
Abstract |
The respiratory tract is one of the major routes of exposure to noxious agents as well as pathogenic particles such as viruses. Natural killer (NK) activity is an important first line of defense to virally infected cells as well as neoplasms, and testing the effects of exposure to toxic compounds on this activity is therefore of importance for the understanding of the immunatoxic potential of the compound. Lymphoid cell suspensions, obtained after enzymatic dispersion of rat lungs and purification over nylon wool calumns showed in vitro natural killer activity towards YAC-lymphoma cells. Validation of the test vich well known NK-activity stimulators as Bacillus Calmette Guerin (BCC), interleukin-2 (IL-2), interferon (IFN) and inhibitors like anti-asiala GM1 antibody confirmed the reliability of the test as an assay for detecting NK-activity in rat lungs. Using this assay, we tested the effects of hexachlorabenzene (HCB), bis(tri-n-buty1tin)oxide (TBTO) and ozone on the NK-activity. Oral exposure to HCB in concentrations of 150 and 450 mg/kg food for six weeks suppressed the NK-activity in rat lungs. This was also true for six weeks of oral exposure of rats to 20 and 80 mg TBTO/kg food, but to a lesser extent. Inhalatory exposure to ozone for 7 days at 0.4-0.8 mg/m* resulted in stimulation, and at 1.6 mg 03/m in suppression of NK-activity. In summary we have developed a reliable method to measure the effects of (toxic) substances on the NK-activity in lungs. |
Supplementary Notes |
The quality of the documents listed in the Office of Toxic Substances database may not meet usual NTIS standards but are included to further the opportunity for the scientific and technical community to locate materials which may not be available elsewhere. The content is the responsibility of OTS. Sponsored by Environmental Protection Agency, Washington, DC. Office of Toxic Substances. |
Availability Notes |
Product reproduced from digital image. Order this product from NTIS by: phone at 1-800-553-NTIS (U.S. customers); (703)605-6000 (other countries); fax at (703)605-6900; and email at orders@ntis.gov. NTIS is located at 5285 Port Royal Road, Springfield, VA, 22161, USA. |
PUB Date Free Form |
13 Oct 1992 |
Category Codes |
57Y; 57U; 68G; 99; 44G |
NTIS Prices |
PC A04/MF A04 |
Document Type |
NT |
Cataloging Source |
NTIS/MT |
Control Number |
018801990 |
Origin |
NTIS |
Type |
CAT |