||Myelin Basic Protein-mRNA Used to Monitor Trimethyltin Neurotoxicity in Rats.
Veronesi, B. ;
Pringle, J. ;
Mezei., C. ;
||Health Effects Research Lab., Research Triangle Park, NC. ;Dalhousie Univ., Halifax (Nova Scotia). Dept. of Biochemistry.
Encephalitogenic basic proteins ;
Messenger RNA ;
RNA probes ;
Nerve cells ;
Nucleic acid hybridization ;
Northern blotting ;
||Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy.
||Trimethyltin (TMT) is an alkyltin that targets neurons of the limbic system. A gene probe (i.e., mRNA) for myelin basic protein (MBP), a major component of central nervous system myelin, was used to monitor this toxic neuropathy in Sprague-Dawley rats. Animals were administered a single intraperitoneal injection of TMT-hydroxide at a neuropathic (8.0 mg/kg/body wt) or nonneuropathic (0.8 mg/kg/body wt) dose and sampled at 1, 3, or 7 days postexposure to correlate the progression of hippocampal neuropathology with probe (i.e., MBP-mRNA) levels. Microscopic examination of the brain showed only moderate but progressive damage over the 7-day postexposure period in animals treated with the neuropathic dose. Neuronal loss was first observed in the dendate gyrus and CA4 at 1 day postexposure, and progressed to the CA3c sector at 3 and 7 days postexposure. Elsewhere in the brain, minimal involvement of the entorhinal cortex neurons occurred 3 days postexposure and intensified by 7 days. No histological damage was seen at the nonneuropathic (0.8 mg/kg) dose. (Copyright (c) 1991 by Academic Press, Inc.)
||Pub. in Toxicology and Applied Pharmacology, v108 n3 p428-435 May 91. Portions of this document are not fully legible. Prepared in cooperation with Dalhousie Univ., Halifax (Nova Scotia). Dept. of Biochemistry.
|NTIS Title Notes
||Reprint: Myelin Basic Protein-mRNA Used to Monitor Trimethyltin Neurotoxicity in Rats.
||PC A03/MF A01