Main Title |
Rapid Test for Chitinase Activity That Uses 4-Methylumbelliferyl-nu-Acetyl-beta-D-Glucosaminide. |
Author |
O'Brien, M. ;
Colwell, R. R. ;
|
CORP Author |
Maryland Univ., College Park. Dept. of Microbiology.;Environmental Research Lab., Gulf Breeze, FL. |
Year Published |
1987 |
Report Number |
EPA/600/J-87/146; |
Stock Number |
PB88-149000 |
Additional Subjects |
Chitinase ;
Biodeterioration ;
Bacteria ;
Microbiology ;
Substrates ;
Polysaccharides ;
Ecology ;
Biochemistry ;
Reprints ;
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
NTIS |
PB88-149000 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Collation |
5p |
Abstract |
One hundred and one strains of bacteria from environmental and clinical sources, most of which were Gram negative, were tested for n-acetyl-Beta-D-glucosaminidase activity using a filter paper spot test with 4-methylumbelliferyl-N-acetyl-Beta-D-glucosaminide (4-MNABetaG) as substrate. The results were compared to those obtained by a conventional plate method for chitinase activity using colloidal chitin as substrate. There was excellent agreement in results for both methods. The filter paper spot test with 4-MNABeta has the advantages of being rapid, simple-to-perform and inexpensive. This method should be adaptable to a wider range of microorganisms, particularly those with unusual growth requirements. (Copyright (c) 1987 American Society for Microbiology.) |
Supplementary Notes |
Pub. in Applied and Environmental Microbiology, v53 n7 p1718-1720 Jul 87. Sponsored by Environmental Research Lab., Gulf Breeze, FL. |
NTIS Title Notes |
Journal article. |
Title Annotations |
Reprint: Rapid Test for Chitinase Activity That Uses 4-Methylumbelliferyl-nu-Acetyl-beta-D-Glucosaminide. |
PUB Date Free Form |
c1987 |
Category Codes |
57K; 68D |
NTIS Prices |
PC A02/MF A01 |
Primary Description |
600/04 |
Document Type |
NT |
Cataloging Source |
NTIS/MT |
Control Number |
808420507 |
Origin |
NTIS |
Type |
CAT |